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Literature summary for 2.7.11.18 extracted from
- Purification and properties of myosin light chain kinases (1991), Methods Enzymol., 196, 34-47.
General Stability
| General Stability | Organism |
|---|---|
| repeated freeze-thawing decreases activity | Meleagris gallopavo |
| repeated freeze-thawing decreases activity | Homo sapiens |
| repeated freeze-thawing decreases activity | Rattus norvegicus |
| repeated freeze-thawing decreases activity | Sus scrofa |
| repeated freeze-thawing decreases activity | Bos taurus |
| repeated freeze-thawing decreases activity | Oryctolagus cuniculus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| phosphorylation | - |
Bos taurus | |
| phosphorylation | - |
Homo sapiens | |
| phosphorylation | - |
Meleagris gallopavo | |
| phosphorylation | - |
Oryctolagus cuniculus | |
| phosphorylation | - |
Rattus norvegicus | |
| phosphorylation | - |
Sus scrofa |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| myofibril | associated | Meleagris gallopavo | 30016 | - |
| myofibril | associated | Homo sapiens | 30016 | - |
| myofibril | associated | Rattus norvegicus | 30016 | - |
| myofibril | associated | Sus scrofa | 30016 | - |
| myofibril | associated | Bos taurus | 30016 | - |
| myofibril | associated | Oryctolagus cuniculus | 30016 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | - |
Meleagris gallopavo |  |
| Ca2+ | - |
Homo sapiens | |
| Ca2+ | - |
Rattus norvegicus | |
| Ca2+ | - |
Sus scrofa | |
| Ca2+ | - |
Bos taurus | |
| Ca2+ | - |
Oryctolagus cuniculus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Meleagris gallopavo | - |
ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | Homo sapiens | - |
ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | Rattus norvegicus | - |
ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | Sus scrofa | - |
ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | Bos taurus | - |
ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | Oryctolagus cuniculus | - |
ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Homo sapiens | - |
- |
- |
| Meleagris gallopavo | - |
- |
- |
| Oryctolagus cuniculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
| Sus scrofa | - |
- |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Meleagris gallopavo |
- |
Homo sapiens |
- |
Bos taurus |
- |
Oryctolagus cuniculus |
| partial | Rattus norvegicus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Sus scrofa | - |
| brain | - |
Bos taurus | - |
| gizzard | - |
Meleagris gallopavo | - |
| myometrium | - |
Sus scrofa | - |
| pancreas | - |
Rattus norvegicus | - |
| platelet | - |
Homo sapiens | - |
| skeletal muscle | - |
Meleagris gallopavo | - |
| skeletal muscle | - |
Homo sapiens | - |
| skeletal muscle | - |
Rattus norvegicus | - |
| skeletal muscle | - |
Sus scrofa | - |
| skeletal muscle | - |
Bos taurus | - |
| skeletal muscle | back and hindlegs | Oryctolagus cuniculus | - |
| smooth muscle | - |
Meleagris gallopavo | - |
| smooth muscle | - |
Homo sapiens | - |
| smooth muscle | - |
Rattus norvegicus | - |
| smooth muscle | - |
Sus scrofa | - |
| smooth muscle | - |
Oryctolagus cuniculus | - |
| smooth muscle | arterial | Bos taurus | - |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1.8 | - |
- |
Bos taurus |
| 6 | - |
- |
Meleagris gallopavo |
| 7.9 | - |
- |
Sus scrofa |
| 7.9 | - |
- |
Bos taurus |
| 24 | - |
skeletal muscle | Oryctolagus cuniculus |
Storage Stability
| Storage Stability | Organism |
|---|---|
| -20°C, in 5% w/v sucrose, several weeks | Oryctolagus cuniculus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | - |
Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | - |
Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | - |
Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | - |
Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | - |
Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | - |
Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of cardiac muscle | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of cardiac muscle | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of cardiac muscle | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of cardiac muscle | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of cardiac muscle | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of cardiac muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Thr-residues in smooth muscle and pancreas myosin light chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-residues in smooth and skeletal muscle | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-residues in smooth and skeletal muscle | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-residues in smooth and skeletal muscle | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-residues in smooth and skeletal muscle | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-residues in smooth and skeletal muscle | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-residues in smooth and skeletal muscle | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | highly specific for regulatory or P-light-chain | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | highly specific for regulatory or P-light-chain | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | highly specific for regulatory or P-light-chain | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | highly specific for regulatory or P-light-chain | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | highly specific for regulatory or P-light-chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | highly specific for regulatory or P-light-chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 18.5 kDa Dictyostelium or 19 kDa skeletal muscle light chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-19 and Thr-18 in smooth muscle myosin light chain | Meleagris gallopavo | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-19 and Thr-18 in smooth muscle myosin light chain | Homo sapiens | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-19 and Thr-18 in smooth muscle myosin light chain | Rattus norvegicus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-19 and Thr-18 in smooth muscle myosin light chain | Sus scrofa | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-19 and Thr-18 in smooth muscle myosin light chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | phosphorylation sites: Ser-19 and Thr-18 in smooth muscle myosin light chain | Oryctolagus cuniculus | ADP + myosin light chain phosphate | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | requirement | Meleagris gallopavo | |
| Calmodulin | requirement | Homo sapiens | |
| Calmodulin | requirement | Rattus norvegicus | |
| Calmodulin | requirement | Sus scrofa | |
| Calmodulin | requirement | Bos taurus | |
| Calmodulin | requirement | Oryctolagus cuniculus | |
| Calmodulin | trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin | Meleagris gallopavo | |
| Calmodulin | trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin | Homo sapiens | |
| Calmodulin | trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin | Rattus norvegicus | |
| Calmodulin | trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin | Sus scrofa | |
| Calmodulin | trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin | Bos taurus | |
| Calmodulin | trypsin or chymotrypsin digested smooth muscle enzyme is independent of Ca2+/calmodulin | Oryctolagus cuniculus | |
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