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Literature summary for 2.7.11.18 extracted from
- Characterization of myosin light-chain kinase from bovine adrenal medulla (1986), Arch. Biochem. Biophys., 245, 379-388.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| additional information | no activation by Ca2+ plus beta-lactoglobulin, cytochrome c, troponin C or parvalbumin | Bos taurus | |
| additional information | no activation by cAMP | Bos taurus |
General Stability
| General Stability | Organism |
|---|---|
| protease inhibitors with broad specificity and glycerol stabilize during initial purification, unstable to further purification | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | at higher free concentrations, 0.4-3 mM, independent of Mg2+ or pH-value | Bos taurus |  |
| EGTA | - |
Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | 0.027 | turkey gizzard myosin light chain | 30°C, pH 7.6, ATP | Bos taurus | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | inhibits at higher free concentrations | Bos taurus | |
| Ca2+ | requirement, only in combination with calmodulin | Bos taurus | |
| Ca2+ | aged enzyme loses Ca2+/calmodulin sensitivity by proteolysis | Bos taurus | |
| Mg2+ | - |
Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 150000 | - |
gel filtration and sedimentation studies | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Bos taurus | involved in regulation of actin-myosin contractile activity in adrenal medulla | ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| adrenal medulla | medulla | Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | acceptor substrates are myosin light chains of skeletal muscle | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | acceptor substrates are myosin light chains of adrenal medullary myosin | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | 1 mol phosphate per mol light chain in skeletal muscle | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | specific for 20 kDa light chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | not 16 kDa light chain | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase b | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | involved in regulation of actin-myosin contractile activity in adrenal medulla | Bos taurus | ADP + myosin light chain phosphate | - |
? | |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | requirement | Bos taurus | |
| Calmodulin | aged enzyme loses Ca2+/calmodulin sensitivity by proteolysis | Bos taurus | |
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Release 2023.1 (January 2023)
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