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Literature summary for 2.7.11.18 extracted from
- Dictyostelium myosin light chain kinase. Purification and characterization (1990), J. Biol. Chem., 265, 13818-13824.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| autophosphorylation | activation | Dictyostelium discoideum | |
| additional information | no activation by cGMP | Dictyostelium discoideum | |
| additional information | no activation by cAMP | Dictyostelium discoideum |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| Histone 2A | - |
Dictyostelium discoideum | |
| Increasing ionic strength | above 0.1 M KCl | Dictyostelium discoideum |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.004 | - |
myosin | myosin from Dictyostelium | Dictyostelium discoideum | |
| 0.004 | - |
Dictyostelium myosin | 22°C, pH 7.5 | Dictyostelium discoideum |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Ca2+ | not | Dictyostelium discoideum |  |
| Ca2+ | calcium/calmodulin regulation | Dictyostelium discoideum | |
| Mg2+ | - |
Dictyostelium discoideum | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
- |
Dictyostelium discoideum |
| 34000 | - |
gel filtration | Dictyostelium discoideum |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + myosin light chain | Dictyostelium discoideum | the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain | ADP + phosphorylated myosin light chain | - |
? | |
| ATP + myosin light chain | Dictyostelium discoideum | - |
ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | Dictyostelium discoideum Ax-3 | the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain | ADP + phosphorylated myosin light chain | - |
? | |
| ATP + myosin light chain | Dictyostelium discoideum Ax-3 | - |
ADP + myosin light chain phosphate | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Dictyostelium discoideum | P25323 | - |
- |
| Dictyostelium discoideum Ax-3 | P25323 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Dictyostelium discoideum |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 0.00615 | - |
- |
Dictyostelium discoideum |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + Dictyostelium myosin | - |
Dictyostelium discoideum | ADP + Dictyostelium myosin phosphate | - |
? | |
| ATP + myosin | - |
Dictyostelium discoideum | ADP + myosin phosphate | - |
? | |
| ATP + myosin light chain | the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain | Dictyostelium discoideum | ADP + phosphorylated myosin light chain | - |
? | |
| ATP + myosin light chain | the enzyme phosphorylates the 18000 Da Dictyostelium myosin regulatory light chain | Dictyostelium discoideum Ax-3 | ADP + phosphorylated myosin light chain | - |
? | |
| ATP + myosin light chain | - |
Dictyostelium discoideum | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrates are myosin heavy chain and phosvitin | Dictyostelium discoideum | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is histone 2-A | Dictyostelium discoideum | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is casein | Dictyostelium discoideum | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | not 16 kDa light chain | Dictyostelium discoideum | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrate is phosphorylase b | Dictyostelium discoideum | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | - |
Dictyostelium discoideum Ax-3 | ADP + myosin light chain phosphate | - |
? | |
| ATP + myosin light chain | no substrates are myosin heavy chain and phosvitin | Dictyostelium discoideum Ax-3 | ADP + myosin light chain phosphate | - |
? | |
| ATP + protein | intramolecular autophosphorylation activates the enzyme | Dictyostelium discoideum | ADP + phosphoprotein | - |
? | |
| ATP + protein | intramolecular autophosphorylation activates the enzyme | Dictyostelium discoideum Ax-3 | ADP + phosphoprotein | - |
? | |
| additional information | skeletal, gizzard smooth and cardiac enzymes perform intramolecular autophosphorylation in the absence of acceptor substrate | Dictyostelium discoideum | ? | - |
? | |
| additional information | skeletal, gizzard smooth and cardiac enzymes perform intramolecular autophosphorylation in the absence of acceptor substrate | Dictyostelium discoideum Ax-3 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| monomer | 1 * 34000, SDS-PAGE | Dictyostelium discoideum |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| myosin light chain kinase | - |
Dictyostelium discoideum |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 22 | - |
assay at | Dictyostelium discoideum |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| Calmodulin | not | Dictyostelium discoideum | |
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