Activating Compound | Comment | Organism | Structure |
---|---|---|---|
Calmodulin | dependent on, activates the nonphosphorylated CaMKIV, residues F320, N321, D322, D323, N318, N324, N330, and N336 are involved in binding, the autoinhibitory domain harbors the binding site for Ca2+ and calmodulin | Homo sapiens | |
ionomycin | activates nonphosphorylated CaMKIV by increasing intracellular Ca2+ concentration | Homo sapiens | |
additional information | Ca2+/calmodulin-bound CaMKIV is further activated by phosphorylation through Ca2+/calmodulin-dependent protein kinase kinase CaMKKbeta, which renders CaMKIV independent on Ca2+ and calmodulin, autonomous activity | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of FLAG-tagged CaMKIV in 293A cells | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
F320D/N321D | site-directed mutagenesis, the mutation changes the sequence FNDD to DDDD, the mutant shows no binding of calmodulin and increased activity in nonphosphorylated status compared to the wild-type enzyme | Homo sapiens |
additional information | mutation of residues N300, N306, N312, N318, N324, N330, N336, N341, and N347 for determination of residues involved in calmodulin binding, no binding of calmodulin and increased activity compared to the wild-type enzyme by nonphosphorylated mutants of N318, N324, N330, and N336, overview | Homo sapiens |
T200A | site-directed mutagenesis, the mutant CaMKIV is not phosphorylated by CaMKKbeta and is thus incapable of developing autonomous activity and control gene transcription, the same effect occurs due to combination of T200A with calmodulin binding site mutations, overview | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | CaMKIV contains an autoinhibitory domain which harbors the binding site for Ca2+ and calmodulin | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics for binding of calmodulin | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | dependent on, activates the nonphosphorylated CaMKIV, the autoinhibitory domain harbors the binding site for Ca2+ and calmodulin | Homo sapiens | |
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Homo sapiens | the enzyme is regulated by Ca2+/calmodulin binding and reversible phosphorylation, overview, the autonomous, Ca2+/calmodulin-independent activity of autophosphorylated Ca2+/calmodulin-dependent protein kinase IV is required for its role in transcription | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | Ca2+/calmodulin-bound CaMKIV is further activated by phosphorylation at Thr200 through Ca2+/calmodulin-dependent protein kinase kinase CaMKKbeta, which renders CaMKIV independent on Ca2+ and calmodulin, autonomous activity, deactivation by dephosphorylation through PP2A | Homo sapiens |
Purification (Comment) | Organism |
---|---|
recombinant FLAG-tagged CaMKIV from 293A cells | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.004 | 0.022 | recombinant nonphosphorylated, calmodulin-binding-residue mutants | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | the enzyme is regulated by Ca2+/calmodulin binding and reversible phosphorylation, overview, the autonomous, Ca2+/calmodulin-independent activity of autophosphorylated Ca2+/calmodulin-dependent protein kinase IV is required for its role in transcription | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
calcium/calmodulin-dependent protein kinase IV | - |
Homo sapiens |
CaMKIV | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |