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Literature summary for 2.7.11.16 extracted from

  • Allen, S.J.; Parthasarathy, G.; Darke, P.L.; Diehl, R.E.; Ford, R.E.; Hall, D.L.; Johnson, S.A.; Reid, J.C.; Rickert, K.W.; Shipman, J.M.; Soisson, S.M.; Zuck, P.; Munshi, S.K.; Lumb, K.J.
    Structure and function of the hypertension variant A486V of G protein-coupled receptor kinase 4 (2015), J. Biol. Chem., 290, 20360-20373.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.11.16

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Sf21 insect cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
mutant enzyme A486V crystallized in the presence of 5'-adenylyl beta,gamma-imidodiphosphate, sitting drop vapor diffusion method, at 4°C in 0.1 M sodium citrate, pH 5.0-6.5, 15-27.5% (w/v) polyethylene glycol 3350 Homo sapiens

Protein Variants

Protein Variants Comment Organism
A142V the mutation is associated with hypertension Homo sapiens
A486V the mutation is associated with hypertension. There is an increased rate of autophosphorylation of a number of residues in the mutant relative to the wild type enzyme Homo sapiens
R65L the mutation is associated with hypertension Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [G-protein-coupled receptor] Homo sapiens
-
 ADP + [G-protein-coupled receptor]phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens P32298
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography, HiTrap SP column chromatography, HiTrap heparin column chromatography and Superdex 75 gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [G-protein-coupled receptor]
-
 Homo sapiens ADP + [G-protein-coupled receptor]phosphate
-
 ?
additional information autophosphorylation of wild type isoform GRK4alpha is required for full kinase activity Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
G protein-coupled receptor kinase 4
-
 Homo sapiens
GRK4
-
 Homo sapiens