Activating Compound | Comment | Organism | Structure |
---|---|---|---|
methacholine | stimulation of M3 muscarinic acetylcholine receptor phosphorylation is reversible by atropine | Homo sapiens | |
additional information | GRK6 activity on the M3 muscarinic acetylcholine receptor is not affected by phorbol-12,13-dibutyrate and Ro 31-8220 | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
stable overexpression of wild-type and K215R mutant GRK6 in SH-SY5Y neuroblastoma cells via adenovirus infection system | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
K215R | site-directed mutagenesis, inactive, dominant negative mutant of GRK6, 30fold overexpression in SH-SY5Y cells produces a 50% suppression of endogenous M3 muscarinic acetylcholine receptor phosphorylation and desensitization by the wild-type enzyme, GRK5 K215R mutant overexpression has no effect | Homo sapiens |
additional information | overexpression of wild-type GRK6 increase the endogenous M3 muscarinic acetylcholine receptor phosphorylation and desensitization level | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
additional information | GRK6 activity on the M3 muscarinic acetylcholine receptor is not affected by phorbol-12,13-dibutyrate and Ro 31-8220 | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [M3 muscarinic acetylcholine receptor] | Homo sapiens | GRK6 plays a major role in specific M3 muscarinic acetylcholine receptor regulation | ADP + phospho-[M3 muscarinic acetylcholine receptor] | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
SH-SY5Y cell | neuroblastoma cell line, contains no GRK5 | Homo sapiens | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + [M3 muscarinic acetylcholine receptor] | - |
Homo sapiens | ADP + phospho-[M3 muscarinic acetylcholine receptor] | - |
? | |
ATP + [M3 muscarinic acetylcholine receptor] | GRK6 plays a major role in specific M3 muscarinic acetylcholine receptor regulation | Homo sapiens | ADP + phospho-[M3 muscarinic acetylcholine receptor] | - |
? |
Synonyms | Comment | Organism |
---|---|---|
G protein-coupled receptor kinase 6 | - |
Homo sapiens |
GRK6 | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |