Cloned (Comment) | Organism |
---|---|
carboxyl-terminus is expressed as a Flag-tagged betaARKct (GRK2 amino acids 495-689) in HeLa and HEK293 cells | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
DELTA495-689 | carboxyl-terminus is expressed as a Flag-tagged betaARKct (GRK2 amino acids 495-689) in HeLa and HEK293 cells | Bos taurus |
S670A | cells overexpressing the S670A-GRK2 mutant have less H2O2-induced death as determined by the terminal deoxynucleotidyl transferase dUTP nick end labeling assay. In cells expressing mutant GRK2, the amount of cell death is significantly reduced by 36.4%. Mutant displays significantly less Hsp90 binding in myocytes after oxidative stress compared with wild-type GRK2. Nonphosphorylated mutant S670A is not localized to mitochondria after oxidative stress | Bos taurus |
S670D | mutant shows the highest level of interaction with Hsp90 compared to wild-type and mutant S670A | Bos taurus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
mitochondrion | after myocardial ischemic and oxidative stress there is increased mitochondrial GRK2 translocation | Rattus norvegicus | 5739 | - |
mitochondrion | after myocardial ischemic and oxidative stress there is increased mitochondrial GRK2 translocation | Bos taurus | 5739 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Bos taurus | carboxyl-terminus of GRK2 interacts with Hsp90. Reactive oxygen species (ROS)-mediated mitochrondrial localisation of GRK2 is dependent on Hsp90 binding | ? | - |
? | |
additional information | Rattus norvegicus | GRK2 interacts with Hsp90. Reactive oxygen species (ROS)-mediated mitochrondrial localisation of GRK2 is dependent on Hsp90 binding | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Rattus norvegicus | - |
- |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | - |
Rattus norvegicus |
phosphoprotein | phosphorylated at Ser670 | Bos taurus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
myocyte | - |
Rattus norvegicus | - |
myocyte | - |
Bos taurus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | carboxyl-terminus of GRK2 interacts with Hsp90. Reactive oxygen species (ROS)-mediated mitochrondrial localisation of GRK2 is dependent on Hsp90 binding | Bos taurus | ? | - |
? | |
additional information | GRK2 interacts with Hsp90. Reactive oxygen species (ROS)-mediated mitochrondrial localisation of GRK2 is dependent on Hsp90 binding | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
bARKct | - |
Rattus norvegicus |
bARKct | - |
Bos taurus |
carboxyl-terminus of beta-adrenergic receptor kinase | - |
Rattus norvegicus |
carboxyl-terminus of beta-adrenergic receptor kinase | - |
Bos taurus |
GRK2 | - |
Rattus norvegicus |
GRK2 | - |
Bos taurus |