Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta-arrestin-2 | forms a complex with GRK3 causing G protein-coupled receptor desensitization | Rattus norvegicus |
Cloned (Comment) | Organism |
---|---|
functional expression of HA-tagged wild-type and mutant GRK3, and coexpression with beta-arrestin-2, in Xenopus laevis oocytes via injection of mRNA, leads to induction of maturation of the oocytes | Rattus norvegicus |
Protein Variants | Comment | Organism |
---|---|---|
K220R | site-directed mutagenesis, kinase-dead mutant of GRK3, the mutant is unable to induce germinal vesicle breakdown | Rattus norvegicus |
additional information | truncated GRK3DELTAC lacking the C-terminal Gbetagamma binding domain is unable to induce germinal vesicle breakdown, and is defective in membrane association | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
concanavalin A | inhibitor of clathrin-mediated receptor endocytosis via clathrin binding domain of beta-arrestin-2, inhibitor reduces the GRK3/beta-arrestin-2 induction activity of germinal vesicle breakdown | Rattus norvegicus | |
monodansyl cadaverin | inhibitor of clathrin-mediated receptor endocytosis via clathrin binding domain of beta-arrestin-2, inhibitor reduces the GRK3/beta-arrestin-2 induction activity of germinal vesicle breakdown | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | associated with, membrane association is required for activity on G protein-coupled receptors, and mediated by the C-terminal Gbetagamma binding domain | Rattus norvegicus | 16020 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Rattus norvegicus | |
Mn2+ | - |
Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + G protein-coupled receptor | Rattus norvegicus | - |
ADP + phosphorylated G protein-coupled receptor | - |
? | |
additional information | Rattus norvegicus | GRK3 is essential for induction of germinal vesicle breakdown, GRK3 forms a complex with beta-arrestin-2 causing G protein-coupled receptor desensitization | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-casein | recombinant HA-tagged wild-type and mutant GRK3 | Rattus norvegicus | ADP + phosphorylated beta-casein | - |
? | |
ATP + G protein-coupled receptor | - |
Rattus norvegicus | ADP + phosphorylated G protein-coupled receptor | - |
? | |
ATP + myelin basic protein | recombinant HA-tagged wild-type and mutant GRK3 | Rattus norvegicus | ADP + phosphorylated myelin basic protein | - |
? | |
additional information | GRK3 is essential for induction of germinal vesicle breakdown, GRK3 forms a complex with beta-arrestin-2 causing G protein-coupled receptor desensitization | Rattus norvegicus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | GRK3 wild-type and mutant domain structures, overview | Rattus norvegicus |
Synonyms | Comment | Organism |
---|---|---|
G protein-coupled receptor kinase 3 | - |
Rattus norvegicus |
GRK3 | - |
Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
22 | - |
assay at room temperature | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.3 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Rattus norvegicus |