Activating Compound | Comment | Organism | Structure |
---|---|---|---|
beta2-adrenergic receptor | interaction of beta-ARK 1 with the agonist-occupied receptor activates | Bos taurus | |
cardiolipin | activates, phosphorylation of beta2-AR | Bos taurus | |
G protein betagamma-subunit | - |
Bos taurus | |
isoproterenol | stimulates | Bos taurus | |
phosphatidic acid | activates, phosphorylation of beta2-AR | Bos taurus | |
phosphatidylglycerol | activates, phosphorylation of beta2-AR | Bos taurus | |
phosphatidylinositol | activates, phosphorylation of beta2-AR, 6fold activation of phosphorylation of RRREEEEESAAA | Bos taurus | |
phosphatidylserine | activates, phosphorylation of beta2-AR | Bos taurus | |
Phospholipid | required for phosphorylation of beta2-AR, activation is associated with a conformational change in beta-ARK 1, acidic phospholipid specificity, not activated by phosphatidylinositol 4,5-diphosphate, direct regulation of beta-ARK 1 activity by phospholipids | Bos taurus |
Cloned (Comment) | Organism |
---|---|
beta-ARK 1 is cloned and expressed in SF9 cells using the baculovirus expression system | Bos taurus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
heparin | potent inhibitor of beta-ARK 1 | Bos taurus | |
phosphatidylinositol 4,5-bisphosphate | inhibits receptor phosphorylation, causes membrane association, 30% inhibition of phosphorylation of RRREEEEESAAA | Bos taurus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.34 | - |
RRREEEEESAAA | pH 7.4, 30°C, recombinant beta-ARK 1 | Bos taurus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
Western blot analysis | Bos taurus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-adrenergic receptor | Bos taurus | agonist-occupied form of the receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | Bos taurus | involved in homologous desensitization of beta-adrenergic receptor | ADP + phospho-beta-adrenergic receptor | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
beta-ARK 1 | - |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | no autophosphorylation of beta-ARK 1 | Bos taurus |
Purification (Comment) | Organism |
---|---|
recombinant beta-ARK 1 expressed in Sf9 cells | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + beta-adrenergic receptor | beta2-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | specifically phosphorylates the agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | incorporation of up to 5 mol phosphate/mol receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | beta-AR from hamster lung | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + beta-adrenergic receptor | involved in homologous desensitization of beta-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
ATP + RRREEEEESAAA | nonreceptor peptide substrate | Bos taurus | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Bos taurus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus |