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Literature summary for 2.7.11.15 extracted from
- Beta-adrenergic receptor kinase. Agonist-dependent receptor binding promotes kinase activation (1993), J. Biol. Chem., 268, 7825-7831.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| beta2-adrenergic receptor | interaction of enzyme with human agonist-occupied receptor specifically and significantly enhances peptide phosphorylation, at lower concentrations, enhances both the affinity and catalytic efficiency for peptide phosphorylation | Bos taurus | |
| rhodopsin | interaction of enzyme with light-activated rhodopsin or truncated rhodopsin lacking its C-terminal phosphorylation sites activates peptide phosphorylation, at lower concentrations, enhances both the affinity and catalytic efficiency for peptide phosphorylation, but intact light-activated rhodopsin slightly inhibits the phosphorylation of RRREEEEESAAA | Bos taurus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| beta-ARK 1 is cloned and expressed in SF9 cells using the baculovirus expression system | Bos taurus |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| rhodopsin | intact light-activated rhodopsin slightly inhibits phosphorylation of RRREEEEESAAA | Bos taurus | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| additional information | - |
additional information | kinetics of peptide phosphorylation, influence of activated rhodopsin and beta2-AR on the Km value for different peptide substrates | Bos taurus | |
| 0.72 | - |
RRREEEEESAAA | pH 7.4, 30°C, recombinant beta-ARK 1 | Bos taurus | |
| 3.3 | - |
RRRASAAASAA | pH 7.4, 30°C, recombinant beta-ARK 1 | Bos taurus | |
| 3.4 | - |
RRRASpASAA | pH 7.4, 30°C, recombinant beta-ARK 1 | Bos taurus | |
| 4.6 | - |
RRRASpAAASAA | pH 7.4, 30°C, recombinant beta-ARK 1 | Bos taurus | |
| 5.1 | - |
RRRAEASAA | pH 7.4, 30°C, recombinant beta-ARK 1 | Bos taurus | |
| 5.4 | - |
RRRASASAA | pH 7.4, 30°C, recombinant beta-ARK 1 | Bos taurus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-adrenergic receptor | Bos taurus | natural substrate: beta2-adrenergic receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | Bos taurus | agonist-occupied form of the receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | Bos taurus | involved in homologous desensitization of beta-adrenergic receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
beta-ARK 1 | - |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant beta-ARK 1 expressed in Sf9 cells | Bos taurus |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| 1 | - |
about, recombinant beta-ARK 1, expressed in SF9 cells | Bos taurus |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-adrenergic receptor | specifically phosphorylates the agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | human beta2-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | beta-ARK 1, substrate specificity, the overall topological structure of the activated receptor plays a key role in regulating signal-dependent receptor phosphorylation | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | natural substrate: beta2-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | involved in homologous desensitization of beta-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + peptide | beta-ARK 1 prefers peptides containing acidic residues on the N-terminal side of a serine or threonine, presence of activated receptor enhances peptide phosphorylation | Bos taurus | ADP + phosphopeptide | - |
? | |
| ATP + peptide | synthetic | Bos taurus | ADP + phosphopeptide | - |
? | |
| ATP + rhodopsin | light-dependent, actual substrate: light-bleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | in form of bovine rod outer segments | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + RRRAEAAASAAA | - |
Bos taurus | ADP + RRRAEAAApSAAA | - |
? | |
| ATP + RRRAEASAA | poor peptide substrate | Bos taurus | ADP + RRRAEApSAA | - |
? | |
| ATP + RRRASAAASAA | poor peptide substrate | Bos taurus | ? | - |
? | |
| ATP + RRRASASAA | poor peptide substrate | Bos taurus | ? | - |
? | |
| ATP + RRRASpAAASAA | poor peptide substrate, higher catalytic efficiency than RRRASAAASAA | Bos taurus | ADP + RRRASpAAASpAA | - |
? | |
| ATP + RRRASpASAA | poor peptide substrate | Bos taurus | ? | - |
? | |
| ATP + RRREEEEESAAA | good peptide substrate, but the activated receptor is a much better substrate | Bos taurus | ? | - |
? | |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bos taurus | |
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Release 2023.1 (January 2023)
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