Any feedback?
Literature summary for 2.7.11.15 extracted from
- Beta-adrenergic receptor kinase: primary structure delineates a multigene family (1989), Science, 246, 235-240.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| isoproterenol | requirement, beta-agonist, beta-adrenergic receptor as substrate | Bos taurus |  |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| cDNA encoding beta-ARK is cloned from brain, sequenced and expressed in COS-7 cells, cDNA encodes a 689 amino acids protein | Bos taurus |
| expression in COS-7 cells | Bos taurus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | requirement | Bos taurus | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 79700 | - |
x * 79700, calculated from the amino acid sequence | Bos taurus |
| 80000 | - |
x * 80000, SDS-PAGE | Bos taurus |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-adrenergic receptor | Bos taurus | agonist-occupied form of the receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | Bos taurus | involved in homologous desensitization of beta-adrenergic receptor | ADP + phospho-beta-adrenergic receptor | - |
? | |
| additional information | Bos taurus | enzyme is important in mediating rapid agonist-specific desensitization | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Bos taurus | - |
- |
- |
| Bos taurus | P21146 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| about 20000fold, from brain | Bos taurus |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| brain | - |
Bos taurus | - |
| brain | cerebral cortex | Bos taurus | - |
| brain | highest beta-ARK mRNA concentrations in brain and spleen, brain: highest levels in cerebral cortex and cerebellum, with significant lower levels in basal ganglia, brain stem, pituitary and hypothalamus | Bos taurus | - |
| cerebellum | brain: highest beta-ARK mRNA concentrations in cerebral cortex and cerebellum | Bos taurus | - |
| cerebral cortex | brain: highest beta-ARK mRNA concentrations in cerebral cortex and cerebellum | Bos taurus | - |
| heart | about 40% of beta-ARK mRNA concentration in brain | Bos taurus | - |
| kidney | about 20% of beta-ARK mRNA concentration in brain | Bos taurus | - |
| lung | about 40% of beta-ARK mRNA concentration in brain | Bos taurus | - |
| additional information | tissue distribution | Bos taurus | - |
| additional information | not in liver, muscle and adrenal gland | Bos taurus | - |
| ovary | about 20% of beta-ARK mRNA concentration in brain | Bos taurus | - |
| spleen | highest beta-ARK mRNA concentrations in brain and spleen | Bos taurus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + beta-adrenergic receptor | beta2-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | specifically phosphorylates the agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | beta-AR from hamster lung | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | beta-AR is a much better substrate than rhodopsin | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | agonist-occupied form of the receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + beta-adrenergic receptor | involved in homologous desensitization of beta-adrenergic receptor | Bos taurus | ADP + phospho-beta-adrenergic receptor | - |
? | |
| ATP + rhodopsin | beta-AR is a much better substrate than rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | light-dependent, actual substrate: light-bleached rhodopsin | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| ATP + rhodopsin | in form of bovine rod outer segments | Bos taurus | ADP + phosphorhodopsin | - |
? | |
| additional information | domain structure | Bos taurus | ? | - |
? | |
| additional information | enzyme is important in mediating rapid agonist-specific desensitization | Bos taurus | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 80000, SDS-PAGE | Bos taurus |
| ? | x * 79700, calculated from the amino acid sequence | Bos taurus |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| beta-adrenergic receptor kinase | - |
Bos taurus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Bos taurus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Bos taurus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Bos taurus | |
Use of this online version of BRENDA is free under the CC BY 4.0 license. See terms of use for full details.
Release 2023.1 (January 2023)
Legal
Curated at
Member of
