Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,2-dioctanoyl-sn-glycerol | - |
Mus musculus | |
1-stearoyl-2-arachidonoyl-sn-glycerol | - |
Mus musculus | |
phorbol 12,13-dibutyrate | - |
Mus musculus |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli as a GST-fusion protein | Mus musculus |
Crystallization (Comment) | Organism |
---|---|
crystal structure of PKCthetaC1B is determined to 1.63 A resolution. It is shown that, Trp253 at the rim of the activator-binding pocket is orientated towards the membrane | Mus musculus |
Protein Variants | Comment | Organism |
---|---|---|
L255G | mutated activator-binding site: mutation reduces affinity for both phorbol ester and diacylglycerol. Mutation causes significantly reduced membrane translocation by phorbol ester treatment | Mus musculus |
Q258G | mutated activator-binding site: mutation reduces affinity for both phorbol ester and diacylglycerol. Mutation causes significantly reduced membrane translocation by phorbol ester treatment | Mus musculus |
T243A | mutated activator-binding site: mutation reduces affinity for both phorbol ester and diacylglycerol. Mutation causes significantly reduced membrane translocation by phorbol ester treatment | Mus musculus |
W253G | mutated activator-binding site: mutation reduces affinity for both phorbol ester and diacylglycerol. Mutation causes significantly reduced membrane translocation by phorbol ester treatment | Mus musculus |
Y239A | mutated activator-binding site: mutation reduces affinity for both phorbol ester and diacylglycerol. Mutation causes significantly reduced membrane translocation by phorbol ester treatment | Mus musculus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
7000 | - |
SDS-PAGE, PKCthetaC1B subdomain | Mus musculus |
100000 | - |
SDS-PAGE, full-length PKCtheta | Mus musculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
using affinity purification through glutathione-Sepharose 4B columns, subsequent cleavage of the GST tag, ammonium sulfate precipitation and gel filtration chromatography | Mus musculus |
Synonyms | Comment | Organism |
---|---|---|
PKCthetaC1B | C1B subdomain | Mus musculus |