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Literature summary for 2.7.11.13 extracted from

  • Grodsky, N.; Li, Y.; Bouzida, D.; Love, R.; Jensen, J.; Nodes, B.; Nonomiya, J.; Grant, S.
    Structure of the catalytic domain of human protein kinase C beta II complexed with a bisindolylmaleimide inhibitor (2006), Biochemistry, 45, 13970-13981.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
1,2-dioleoyl-sn-glycerol activates Homo sapiens
12-O-Tetradecanoylphorbol 13-acetate activates Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of full-length His-tagged isoform PKCbetaII in Spodoptera frugiperda Sf21 cells Homo sapiens

Crystallization (Commentary)

Crystallization (Comment) Organism
purified recombinant isozyme PKCbetaII catalytic domain, residues 321-673, hanging drop vapour diffusion method, 8 mg/ml protein in 0.1 M acetamidoiminodiacetic acid, pH 6.5, and 1.7-2.3 M sodium acetate, 19°C, X-ray diffraction structure determination and analysis at 3.2 A resolution, molecular replacement, modeling Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
3-(1-(3-(dimethylamino)propyl)-2-methyl-1H-indol-3-yl)-4-(2-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione a bisindolylmaleimide analogue of BIM-1 inhibitor Homo sapiens

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics Homo sapiens

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+
-
Homo sapiens
Mg2+
-
Homo sapiens

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
isoform PKCbetaII
-

Purification (Commentary)

Purification (Comment) Organism
recombinant full-length enzyme by nickel affinity chromatography, proteolytic clevage of the recombinant full-length enzyme to obtain the catalytic domain, followed by anion exchange chromatography and gel filtration Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + RFARKGSLRQKNV a commercially available peptide substrate Homo sapiens ADP + phoshorylated RFARKGSLRQKNV
-
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Synonyms

Synonyms Comment Organism
PKC
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens