Activating Compound | Comment | Organism | Structure |
---|---|---|---|
1,2-dioleoyl-sn-glycerol | activates | Homo sapiens | |
12-O-Tetradecanoylphorbol 13-acetate | activates | Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expression of full-length His-tagged isoform PKCbetaII in Spodoptera frugiperda Sf21 cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
purified recombinant isozyme PKCbetaII catalytic domain, residues 321-673, hanging drop vapour diffusion method, 8 mg/ml protein in 0.1 M acetamidoiminodiacetic acid, pH 6.5, and 1.7-2.3 M sodium acetate, 19°C, X-ray diffraction structure determination and analysis at 3.2 A resolution, molecular replacement, modeling | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3-(1-(3-(dimethylamino)propyl)-2-methyl-1H-indol-3-yl)-4-(2-methyl-1H-indol-3-yl)-1H-pyrrole-2,5-dione | a bisindolylmaleimide analogue of BIM-1 inhibitor | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
additional information | - |
additional information | kinetics | Homo sapiens |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | - |
Homo sapiens | |
Mg2+ | - |
Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
isoform PKCbetaII | - |
Purification (Comment) | Organism |
---|---|
recombinant full-length enzyme by nickel affinity chromatography, proteolytic clevage of the recombinant full-length enzyme to obtain the catalytic domain, followed by anion exchange chromatography and gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RFARKGSLRQKNV | a commercially available peptide substrate | Homo sapiens | ADP + phoshorylated RFARKGSLRQKNV | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKC | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |