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Literature summary for 2.7.11.13 extracted from

  • Uchino, M.; Sakai, N.; Kashiwagi, K.; Shirai, Y.; Shinohara, Y.; Hirose, K.; Iino, M.; Yamamura, T.; Saito, N.
    Isoform-specific phosphorylation of metabotropic glutamate receptor 5 by protein kinase C (PKC) blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKC (2004), J. Biol. Chem., 279, 2254-2261.
    View publication on PubMed
Search for more literature for 2.7.11.13

Activating Compound

Activating Compound Comment Organism Structure
additional information PKC activation is influenced by intracellular Ca2+ concentration and activity of the metabotropic glutamate receptor 5 Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of GFP-tagged wild-type PKC isozymes in HEK-293 cells, and of GFP-tagged PKCgamma wild-type and mutants lacking the C1 or C2 domain in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information downregulation of PKCdelta by short interfering RNA, deletion of domain C2 of PKCgamma leads to complete loss of activity, while deletion of C1 domain shows no effect on PKCgamma activity Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
2-methyl-6-(phenylethynyl)-pyridine complete inhibition of PKCgamma oscillation at 0.05 mM Homo sapiens
arachidonoyl fluoromethylketone inhibition of PKCgamma oscillation at 0.1 mM Homo sapiens
bromoerol lactone slight inhibition of PKCgamma oscillation at 0.1 mM Homo sapiens
compound 48/80 complete inhibition of PKCgamma oscillation at 0.1 mM Homo sapiens
cytochalasine D inhibition of PKCgamma oscillation at 0.01 mM Homo sapiens
ethanol 0.03%, inhibition of PKCgamma oscillation at 0.1 mM Homo sapiens
additional information no inhibition of PKCgamma oscillation by EGTA, aristolochic acid, and colchicine Homo sapiens
thapsigargin complete inhibition of PKCgamma oscillation at 0.005 mM Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol translocation of PKCgamma from cytosol to membrane Homo sapiens 5829
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plasma membrane translocation of PKCgamma from cytosol to membrane, PKCgamma oscillates on the membrane induced by glutamate and activated metabotropic glutamate receptor 5, overview Homo sapiens 5886
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Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ dependent on, depends on the isozyme, e.g. isozyme betaII and gamma are dependent on Ca2+, while isozyme delta is not Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + metabotropic glutamate receptor 5 Homo sapiens isozyme-specific phosphorylation of metabotropic glutamate receptor 5 by PKCdelta blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKCgamma ADP + phosphorylated metabotropic glutamate receptor 5
-
 ?
additional information Homo sapiens activation of metabotropic glutamate receptor 5 induces translocation of PKCgamma and PKCdelta to plasma membrane and elicits cyclical translocations of myristoylated alanine-rich PKC substrate from plasma membrane to cytosol ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
 several isozymes
-

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + metabotropic glutamate receptor 5 isozyme-specific phosphorylation of metabotropic glutamate receptor 5 by PKCdelta blocks Ca2+ oscillation and oscillatory translocation of Ca2+-dependent PKCgamma Homo sapiens ADP + phosphorylated metabotropic glutamate receptor 5
-
 ?
ATP + metabotropic glutamate receptor 5 phosphorylation at Thr840, PKCdelta is active with wild-type substrate and mutant T840A, but not with mutant T840D, no phosphorylation by PKCgamma Homo sapiens ADP + phosphorylated metabotropic glutamate receptor 5
-
 ?
additional information activation of metabotropic glutamate receptor 5 induces translocation of PKCgamma and PKCdelta to plasma membrane and elicits cyclical translocations of myristoylated alanine-rich PKC substrate from plasma membrane to cytosol Homo sapiens ?
-
 ?
additional information myristoylated alanine-rich PKC substrate is repetitively phosphorylated by oscillating gammaPKC on the plasma membrane Homo sapiens ?
-
 ?

Synonyms

Synonyms Comment Organism
PKC
-
 Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Homo sapiens