Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli | Plasmodium falciparum |
Crystallization (Comment) | Organism |
---|---|
cyclic nucleotide-binding domain CNB-D in the apo and cGMP bound states, hanging drop vapor diffusion method, using 0.2 M lithium sulfate, 15% ethanol, 0.1 M citrate at pH 5.5 and 10% (w/v) 1,5-diaminopentane dihydrochloride | Plasmodium falciparum |
Protein Variants | Comment | Organism |
---|---|---|
D533A | the mutant shows strongly reduced activity compared to the wild type enzyme | Plasmodium falciparum |
Q532A | the mutant shows strongly reduced activity compared to the wild type enzyme | Plasmodium falciparum |
R484A | the mutant shows strongly reduced activity compared to the wild type enzyme | Plasmodium falciparum |
R484A/Q532A/D533A | the mutant shows strongly reduced activity compared to the wild type enzyme | Plasmodium falciparum |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | Plasmodium falciparum | - |
ADP + phosphorylated protein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Plasmodium falciparum | - |
- |
- |
Purification (Comment) | Organism |
---|---|
IMAC nickel column chromatography and Superdex 75 gel filtration | Plasmodium falciparum |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
merozoite | - |
Plasmodium falciparum | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | - |
Plasmodium falciparum | ADP + phosphorylated protein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKG | - |
Plasmodium falciparum |
General Information | Comment | Organism |
---|---|---|
malfunction | blocking of the enzyme stops both replication and transmission of the Plasmodium falciparum | Plasmodium falciparum |