Literature summary for 2.7.11.12 extracted from

Campbell, J.C.; Kim, J.J.; Li, K.Y.; Huang, G.Y.; Reger, A.S.; Matsuda, S.; Sankaran, B.; Link, T.M.; Yuasa, K.; Ladbury, J.E.; Casteel, D.E.; Kim, C.
Structural basis of cyclic nucleotide selectivity in cGMP-dependent protein kinase II (2016), J. Biol. Chem., 291, 5623-5633.

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Activating Compound

Activating Compound	Comment	Organism	Structure
8-bromo-cGMP	-	Homo sapiens

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3) cells	Homo sapiens

Crystallization (Commentary)

Crystallization (Comment)	Organism
enzyme bound with cGMP or cAMP, vapor diffusion method, using 200 mM sodium malonate at pH 5.0, 5-10% (v/v) ethylene glycol, and 20% (w/v) PEG, or 200 mM calcium chloride, 200 mM cadmium chloride, 200 mM cobalt (II) chloride, and 20% (w/v) PEG 3350	Homo sapiens

Protein Variants

Protein Variants	Comment	Organism
D412A/R415A	the mutant shows reduced activity compared to the wild type enzyme	Homo sapiens

Localization

Localization	Comment	Organism	GeneOntology No.	Textmining
membrane	-	Homo sapiens	16020	-

Organism

Organism	UniProt	Comment	Textmining
Homo sapiens	Q13237	-	-

Purification (Commentary)

Purification (Comment)	Organism
immobilized metal ion affinity column chromatography and Superdex 75 gel filtration	Homo sapiens

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + VASP protein	-	Homo sapiens	ADP + phosphorylated VASP protein	-	?

Synonyms

Synonyms	Comment	Organism
cGMP-dependent protein kinase II	-	Homo sapiens
PKG II	-	Homo sapiens

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Release 2023.1 (January 2023)