Activating Compound | Comment | Organism | Structure |
---|---|---|---|
8-bromo-cGMP | - |
Homo sapiens |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli BL21(DE3) cells | Homo sapiens |
Crystallization (Comment) | Organism |
---|---|
enzyme bound with cGMP or cAMP, vapor diffusion method, using 200 mM sodium malonate at pH 5.0, 5-10% (v/v) ethylene glycol, and 20% (w/v) PEG, or 200 mM calcium chloride, 200 mM cadmium chloride, 200 mM cobalt (II) chloride, and 20% (w/v) PEG 3350 | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
D412A/R415A | the mutant shows reduced activity compared to the wild type enzyme | Homo sapiens |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | - |
Homo sapiens | 16020 | - |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | Q13237 | - |
- |
Purification (Comment) | Organism |
---|---|
immobilized metal ion affinity column chromatography and Superdex 75 gel filtration | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + VASP protein | - |
Homo sapiens | ADP + phosphorylated VASP protein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cGMP-dependent protein kinase II | - |
Homo sapiens |
PKG II | - |
Homo sapiens |