Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cGMP | - |
Mus musculus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
cytosol | PKG Ialpha and Ibeta are largely cytosolic enzymes | Mus musculus | 5829 | - |
membrane | isozyme PKG II | Mus musculus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + BAD protein | Mus musculus | phosphorylation on Ser155 by PKG I | ADP + BAD phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mus musculus | - |
gene prkg1, encoding isozyme PKG I in two splice variants, PKG Ialpha and Ibeta, and gene prkg2 encoding isozyme PKG II | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
MC-3T3 cell | - |
Mus musculus | - |
MLO-Y4 cell | osteocyte-like cells | Mus musculus | - |
osteoblast | primary | Mus musculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + BAD protein | phosphorylation on Ser155 by PKG I | Mus musculus | ADP + BAD phosphoprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PKG | - |
Mus musculus |
PKG I | - |
Mus musculus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Mus musculus |
Organism | Comment | Expression |
---|---|---|
Mus musculus | silencing of PKG I or PKG II by siRNA targeting | down |
General Information | Comment | Organism |
---|---|---|
metabolism | requirement for NO/cGMP/PKG signaling downstream of 17beta-estradiol for prevention of etoposide-induced death of MLO-Y4 osteocyte-like cells. BAD phosphorylation on Ser136 and Ser155 by Akt and PKG I, respectively, regulate BAD interaction with Bcl-2. NO activates soluble guanylate cyclase, generating cGMP, which in turn regulates cGMP-dependent protein kinases and phosphodiesterases. Estradiol-induced Akt and ERK activation in osteocytes is mediated by NO/cGMP/PKG II, and Akt is necessary for the anti-apoptotic effect of estradiol | Mus musculus |
physiological function | PKG II is necessary for BAD Ser136 phosphorylation by Akt, allowing direct phosphorylation of Ser155 by PKG Ialpha. PKGIalpha and PKGII are independently required for estradiol- and cGMP-mediated protection against apoptosis, role of NO/cGMP signaling in 17beta-estradiol regulation of osteocyte survival | Mus musculus |