Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + DAPK2 protein | Homo sapiens | i.e death-associated protein kinase 2, phosphorylation at Ser299, Ser367 and Ser368 type I cGMP-dependent protein kinase | ADP + DAPK2 phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
breast cancer cell | - |
Homo sapiens | - |
MCF-7 cell | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + DAPK2 protein | i.e death-associated protein kinase 2, phosphorylation at Ser299, Ser367 and Ser368 type I cGMP-dependent protein kinase | Homo sapiens | ADP + DAPK2 phosphoprotein | - |
? | |
ATP + DAPK2 protein | i.e death-associated protein kinase 2, phosphorylation at Ser299, Ser367 and Ser368 type I cGMP-dependent protein kinase, recombinant wild-type and mutant FLAG-tagged DAPK2 expressed in COS-7 cells, substrate detection from microarray substrate screening, overview | Homo sapiens | ADP + DAPK2 phosphoprotein | - |
? |
Synonyms | Comment | Organism |
---|---|---|
cGK-I | - |
Homo sapiens |
cGMP-dependent protein kinase I | - |
Homo sapiens |
cGMP-dependent protein kinase-I | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
assay at | Homo sapiens |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
metabolism | importance of the cGK-I/DAPK2 signaling pathway in regulating apoptosis, overview | Homo sapiens |
additional information | consensus motif for cGK is RR/KXS/T | Homo sapiens |
physiological function | cGMP-dependent protein kinase-I (cGK-I) induces apoptosis in various cancer cell lines, and death-associated protein kinase 2, DAPK2, is one of the targets of cGK-I in apoptosis induction. Phosphorylation of DAPK2 at Ser299 by cGKI enhances DAPK2 kinase activity, but overexpression of a phospho-mimic DAPK2 mutant, DAPK2 S299D, as well as mutant S299D/S318E, strongly induce apoptosis in human breast cancer MCF-7 cells compared with wild-type DAPK2, while a S367D/S368D mutant does not | Homo sapiens |