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Literature summary for 2.7.11.12 extracted from
- Autophosphorylation of cGMP-dependent protein kinase type II (2003), J. Biol. Chem., 278, 28651-28658.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| cGMP | activates native and recombinant enzyme | Mus musculus |  |
| cGMP | activates native and recombinant enzyme | Homo sapiens | |
| cGMP | activates native and recombinant enzyme, the activation of recombinant cGKII is reduced because the enzyme already partially phosphorylated | Rattus norvegicus | |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of His-tagged cGKII in Spodoptera frugiperda Sf9 cells using the baculovirus infection system | Mus musculus |
| expression of His-tagged cGKII in Spodoptera frugiperda Sf9 cells using the baculovirus infection system | Homo sapiens |
| expression of wild-type and mutant cGKIIs in COS-1 cells | Rattus norvegicus |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| S110A/S114A/S445A | site-directed mutagenesis, the mutant shows 60% reduced autophosphorylation activity compared to the wild-type GKII | Rattus norvegicus |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Mus musculus | |
| Mg2+ | - |
Homo sapiens | |
| Mg2+ | - |
Rattus norvegicus | |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Mus musculus | the enzyme performs autophosphorylation at serines 110, 114, 117, 126, and 445 and at Thr109 in vitro and in vivo | ? | - |
? | |
| additional information | Homo sapiens | the enzyme performs autophosphorylation at serines 110, 114, 117, 126, and 445 and at Thr109 in vitro and in vivo | ? | - |
? | |
| additional information | Rattus norvegicus | the enzyme performs autophosphorylation at serines 110, 114, 117, 126, and 445 and at Thr109 in vitro and in vivo | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
| Mus musculus | - |
- |
- |
| Rattus norvegicus | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the enzyme performs autophosphorylation at serines 110, 114, 117, 126, and 445 and at Thr109, phosphorylation at Ser110 and Ser97 of the recombinant enzyme expressed in Sf9 insect cells, phosphorylation site mapping | Mus musculus |
| phosphoprotein | the enzyme performs autophosphorylation at serines 110, 114, 117, 126, and 445 and at Thr109, phosphorylation at Ser110 and Ser97 of the recombinant enzyme expressed in Sf9 insect cells, phosphorylation site mapping | Homo sapiens |
| phosphoprotein | the enzyme performs autophosphorylation at serines 110, 114, 117, 126, and 445 and at Thr109, phosphorylation at Ser110 and Ser97 of the recombinant enzyme expressed in Sf9 insect cells, phosphorylation site mapping | Rattus norvegicus |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant His-tagged cGKII from Sf9 cells by nikel affinity chromatography | Mus musculus |
| recombinant His-tagged cGKII from Sf9 cells by nikel affinity chromatography | Homo sapiens |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| epithelium | intestinal | Mus musculus | - |
| epithelium | intestinal | Homo sapiens | - |
| epithelium | intestinal | Rattus norvegicus | - |
| intestine | epithelium | Mus musculus | - |
| intestine | epithelium | Homo sapiens | - |
| intestine | epithelium | Rattus norvegicus | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | the enzyme performs autophosphorylation at serines 110, 114, 117, 126, and 445 and at Thr109 in vitro and in vivo | Mus musculus | ? | - |
? | |
| additional information | the enzyme performs autophosphorylation at serines 110, 114, 117, 126, and 445 and at Thr109 in vitro and in vivo | Homo sapiens | ? | - |
? | |
| additional information | the enzyme performs autophosphorylation at serines 110, 114, 117, 126, and 445 and at Thr109 in vitro and in vivo | Rattus norvegicus | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cGK II | - |
Mus musculus |
| cGK II | - |
Homo sapiens |
| cGK II | - |
Rattus norvegicus |
| cGMP-dependent protein kinase type II | - |
Mus musculus |
| cGMP-dependent protein kinase type II | - |
Homo sapiens |
| cGMP-dependent protein kinase type II | - |
Rattus norvegicus |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Mus musculus |
| 30 | - |
assay at | Homo sapiens |
| 30 | - |
assay at | Rattus norvegicus |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.4 | - |
assay at | Mus musculus |
| 7.4 | - |
assay at | Homo sapiens |
| 7.4 | - |
assay at | Rattus norvegicus |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Mus musculus | |
| ATP | - |
Homo sapiens | |
| ATP | - |
Rattus norvegicus | |
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