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Literature summary for 2.7.11.12 extracted from

  • Hou, Y.; Ye, R.D.; Browning, D.D.
    Activation of the small GTPase Rac1 by cGMP-dependent protein kinase (2004), Cell. Signal., 16, 1061-1069.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
8-Br-cGMP activates Homo sapiens
cGMP dependent on Homo sapiens
additional information enzyme is induced by NO via cGMP Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of GFP-tagged dominant negative mutant type 1-alpha PKG in A-549 cells and of untagged mutant in HEK-293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
additional information the enzyme is inactivated by expression of a dominant negative mutant PKG construct Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information the enzyme is inactivated by expression of a dominant negative mutant type 1alpha PKG construct Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol mainly Homo sapiens 5829
-
extracellular dynamic membrane localization Homo sapiens
-
-
plasma membrane
-
Homo sapiens 5886
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Rac1 Homo sapiens the enzyme activates the recombinant Myc-tagged small GTPase Rac1 in HEK-293 cells leading to activation of PAK1, the enzyme induces phosphorylation of p38 which activates MAPK, regulation, overview ADP + phosphorylated Rac-1
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
type 1alpha PKG
-

Source Tissue

Source Tissue Comment Organism Textmining
fibroblast
-
Homo sapiens
-
HEK-293 cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Rac1 the enzyme activates the recombinant Myc-tagged small GTPase Rac1 in HEK-293 cells leading to activation of PAK1, the enzyme induces phosphorylation of p38 which activates MAPK, regulation, overview Homo sapiens ADP + phosphorylated Rac-1
-
?
ATP + Rac1 no activity with Rac-1 mutant T17N Homo sapiens ADP + phosphorylated Rac-1
-
?

Synonyms

Synonyms Comment Organism
PKG
-
Homo sapiens
type 1alpha PKG
-
Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens