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Literature summary for 2.7.11.11 extracted from

  • Deminoff, S.J.; Howard, S.C.; Hester, A.; Warner, S.; Herman, P.K.
    Using substrate-binding variants of the cAMP-dependent protein kinase to identify novel targets and a kinase domain important for substrate interactions in Saccharomyces cerevisiae (2006), Genetics, 173, 1909-1917.
    View publication on PubMedView publication on EuropePMC
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Protein Variants

Protein Variants Comment Organism
K336A/H338A i.e. Tpk1K336A/H338A, site-directed mutagenesis, a catalytically inactive PKA variant, that exhibits a stable binding to the substrate Saccharomyces cerevisiae
additional information TPK1 overexpression resulted in a severe growth defect Saccharomyces cerevisiae
R324A i.e. Tpk1R324A, site-directed mutagenesis, a catalytically inactive PKA variant, that exhibits a stable binding to the substrate with increased affinity through a conformational change Saccharomyces cerevisiae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
 additional information PKA associates with substrates with a relatively low affinity Saccharomyces cerevisiae

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Saccharomyces cerevisiae

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + Dot6 Saccharomyces cerevisiae a protein implicated in telomere function ADP + phosphorylated Dot6
-
 ?

Organism

Organism UniProt Comment Textmining
Saccharomyces cerevisiae
-
 several strains, overview
-

Reaction

Reaction Comment Organism Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate conserved residues K336 and H338 are important for catalytic activity, R324 is important in substrate binding Saccharomyces cerevisiae
a

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + Dot6 a protein implicated in telomere function Saccharomyces cerevisiae ADP + phosphorylated Dot6
-
 ?
ATP + Dot6 a protein implicated in telomere function, substrate of Tpk1 Saccharomyces cerevisiae ADP + phosphorylated Dot6
-
 ?

Synonyms

Synonyms Comment Organism
PKA
-
 Saccharomyces cerevisiae
Tpk1
-
 Saccharomyces cerevisiae

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Saccharomyces cerevisiae