Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP | dynamics of the binding domain structure, molecular binding mechanism, overview | Bos taurus |
Cloned (Comment) | Organism |
---|---|
expression of wild-type and mutant subunit RIalpha residues 91-244 constructs, containing the minimal motifs for cAMP-dependent tight binding of subunit C, in Escherichia coli strain BL21(DE3) | Bos taurus |
Protein Variants | Comment | Organism |
---|---|---|
I204A | site-directed mutagenesis, subunit RIalpha residue mutation, slightly impaired activation of subunit C, mutant show a slightly lower melting temperature compared to the wild-type subunit RIalpha | Bos taurus |
L203A | site-directed mutagenesis, subunit RIalpha residue mutation, slightly impaired activation of subunit C, mutant show a slightly lower melting temperature compared to the wild-type subunit RIalpha | Bos taurus |
R241A | site-directed mutagenesis, subunit RIalpha residue mutation, slightly impaired activation of subunit C, mutant show a slightly lower melting temperature compared to the wild-type subunit RIalpha | Bos taurus |
Y229A | site-directed mutagenesis, subunit RIalpha residue mutation, slightly impaired activation of subunit C, mutant show a slightly lower melting temperature compared to the wild-type subunit RIalpha | Bos taurus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Bos taurus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Bos taurus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant wild-type and mutant subunit RIalpha residues 91-244 construct dimers from Escherichia coli strain BL21(DE3) by cAMP affinity chromatography, dialysis, and gel filtration | Bos taurus |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate | activation mechanism | Bos taurus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Kemptide | LRRASLG peptide substrate, activity of the catalytic subunit C | Bos taurus | ADP + Kemptide phosphate | - |
? | |
additional information | cAMP binds to the helical subunit C binding regions relayed by the highly dynamic switch of the C-helix of subunit RIalpha, which is linked to cAMP by a salt bridge essential for activation | Bos taurus | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | determination of cAMP binding and interaction structures of subunits C and RIalpha, overview | Bos taurus |
Synonyms | Comment | Organism |
---|---|---|
type I PKA | - |
Bos taurus |
type I protein kinase A | - |
Bos taurus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Bos taurus |