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Literature summary for 2.7.11.11 extracted from

  • Zhang, W.; Morris, G.Z.; Beebe, S.J.
    Characterization of the cAMP-dependent protein kinase catalytic subunit Cgamma expressed and purified from sf9 cells (2004), Protein Expr. Purif., 35, 156-169.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
cAMP dependent on Mus musculus
cAMP dependent on Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged catalytic subunit Calpha in Escherichia coli strain BL21(DE3), expression of catalytic subunit Calpha in Spodoptera frugiperda Sf9 cells via baculovirus infection system Mus musculus
expression of His-tagged catalytic subunit Cgamma in Escherichia coli strain BL21(DE3), stable expression of catalytic subunit Cgamma in cAMP-resistant mouse Y1 adrenal mutant cell line, expression of catalytic subunit Cgamma in Spodoptera frugiperda Sf9 cells via baculovirus infection system Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
additional information no inhibition of catalytic subunit Cgamma by PKI Homo sapiens
PKI catalytic subunit Calpha Mus musculus
pseudosubstrate peptides IC50 values for inhibition of the regulatory subunits of PKA by endogenous inhibitors, overview Homo sapiens
pseudosubstrate peptides IC50 values for inhibition of he regulatory subunits of PKA by endogenous inhibitors, overview Mus musculus

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
additional information
-
additional information kinetics for catalytic subunit Cgamma Mus musculus
additional information
-
additional information kinetics for catalytic subunit Cgamma Homo sapiens
0.0125
-
Kemptide pH 7.0, 30°C, recombinant catalytic subunit Cgamma Homo sapiens
0.0253
-
RFARKGSLREKNV pH 7.0, 30°C, recombinant catalytic subunit Cgamma Homo sapiens
0.0333
-
RKRSRAE pH 7.0, 30°C, recombinant catalytic subunit Cgamma Homo sapiens
0.0333
-
RKRSRKE pH 7.0, 30°C, recombinant catalytic subunit Cgamma Homo sapiens
0.038
-
Kemptide pH 7.0, 30°C, recombinant catalytic subunit Calpha Mus musculus
0.05
-
RFARKGSLREKNV pH 7.0, 30°C, recombinant catalytic subunit Calpha Mus musculus
0.0503
-
RRLSSLRA pH 7.0, 30°C, recombinant catalytic subunit Cgamma Homo sapiens
0.293
-
RKRSRAE pH 7.0, 30°C, recombinant catalytic subunit Calpha Mus musculus
0.338
-
RRLSSLRA pH 7.0, 30°C, recombinant catalytic subunit Calpha Mus musculus
0.5
-
RKRSRKE pH 7.0, 30°C, recombinant catalytic subunit Calpha Mus musculus

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
Mus musculus
Mg2+
-
Homo sapiens
Zn2+
-
Mus musculus
Zn2+
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
41000
-
1 * 41000, isolated recombinant catalytic subunit Cgamma from Sf9 cells, SDS-PAGE Homo sapiens
42000
-
1 * 42000, isolated recombinant catalytic subunit Calpha from Escherichia coli, SDS-PAGE Mus musculus

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-
Mus musculus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant catalytic subunit Calpha from Escherichia coli by nickel affinity chromatography, recombinant catalytic subunit Calpha from Sf9 insect cells 62fold by adsorption chromatography and gel filtration Mus musculus
recombinant His-tagged catalytic subunit Cgamma from Escherichia coli strain BL21(DE3) by nickel affinity chromatography, recombinant catalytic subunit Cgamma 273fold from Sf9 insect cells by adsorption chromatography and gel filtration Homo sapiens

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.31
-
purified recombinant catalytic subunit Cgamma, substrate Kemptide Homo sapiens
0.81
-
purified recombinant catalytic subunit Cgamma, substrate histone Homo sapiens
5.3
-
purified recombinant catalytic subunit Calpha Mus musculus

Storage Stability

Storage Stability Organism
-70°C, purified recombinant catalytic subunit Cgamma, 0.5 M NaCl, 10 mM potassium phosphate, pH 6.9, 1 mM EDTA, 30% glycerol, on freeze-thaw cycle, stable up to one year Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + histone 3s activity of the catalytic subunit Cgamma Homo sapiens ADP + phosphorylated histone 3s
-
?
ATP + histone IIIS activity of the catalytic subunit Cgamma Mus musculus ADP + phosphorylated histone IIIS
-
?
ATP + Kemptide activity of the catalytic subunit Calpha Mus musculus ADP + Kemptide phosphate
-
?
ATP + Kemptide PKA-derived substrate, activity of the catalytic subunit Cgamma Homo sapiens ADP + Kemptide phosphate
-
?
ATP + RFARKGSLREKNV protein kinase C-derived peptide, activity of the catalytic subunit Calpha Mus musculus ADP + RFARKG-phosphoserine-LREKNV
-
?
ATP + RFARKGSLREKNV protein kinase C-derived peptide, activity of the catalytic subunit Cgamma Homo sapiens ADP + RFARKG-phosphoserine-LREKNV
-
?
ATP + RKRSRAE cGPK-1-derived peptide, activity of the catalytic subunit Calpha Mus musculus ADP + RKR-phosphoserine-RAE
-
?
ATP + RKRSRAE cGPK-1-derived peptide, activity of the catalytic subunit Cgamma Homo sapiens ADP + RKR-phosphoserine-RAE
-
?
ATP + RKRSRKE cGPK-2-derived peptide, activity of the catalytic subunit Calpha Mus musculus ADP + RKR-phosphoserine-RKE
-
?
ATP + RKRSRKE cGPK-2-derived peptide, activity of the catalytic subunit Cgamma Homo sapiens ADP + RKR-phosphoserine-RKE
-
?
ATP + RRLSSLRA S6 kinase-derived peptide, activity of the catalytic subunit Calpha Mus musculus ADP + RRL-phosphoserine-phosphoserine-LRA
-
?
ATP + RRLSSLRA S6 kinase-derived peptide, activity of the catalytic subunit Cgamma Homo sapiens ADP + RRL-phosphoserine-phosphoserine-LRA
-
?
additional information substrate specificity, overview Mus musculus ?
-
?
additional information substrate specificity, overview Homo sapiens ?
-
?

Subunits

Subunits Comment Organism
monomer 1 * 41000, isolated recombinant catalytic subunit Cgamma from Sf9 cells, SDS-PAGE Homo sapiens
monomer 1 * 42000, isolated recombinant catalytic subunit Calpha from Escherichia coli, SDS-PAGE Mus musculus
More determination of Stoke's radius of purified recombinant catalytic subunit Calpha Mus musculus
More determination of Stoke's radius of purified recombinant catalytic subunit Cgamma Homo sapiens

Synonyms

Synonyms Comment Organism
PKA
-
Mus musculus
PKA
-
Homo sapiens

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mus musculus
30
-
assay at Homo sapiens

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Mus musculus
7
-
assay at Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mus musculus
ATP
-
Homo sapiens