Literature summary for 2.7.11.11 extracted from

Eyers, P.A.; Liu, J.; Hayashi, N.R.; Lewellyn, A.L.; Gautier, J.; Maller, J.L.
Regulation of the G2/M transition in Xenopus oocytes by the cAMP-dependent protein kinase (2005), J. Biol. Chem., 280, 24339-24346.

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Activating Compound

Activating Compound	Comment	Organism	Structure
cAMP	dependent on	Xenopus laevis

Cloned(Commentary)

Cloned (Comment)	Organism
expression of N-terminally His6-tagged wild-type and mutant catalytic subunits C in Escherichia coli strain BL21(DE3)	Xenopus laevis

Protein Variants

Protein Variants	Comment	Organism
D328A	site-directed mutagenesis of the catalytic subunit, mutant shows decreased interaction with the regulatory RI subunit	Xenopus laevis
D328A/K72H	site-directed mutagenesis of the catalytic subunit, inactive mutant showing decreased interaction with the regulatory RII subunit	Xenopus laevis
K72H	site-directed mutagenesis of the catalytic subunit, inactive mutant	Xenopus laevis
K72R	site-directed mutagenesis of the catalytic subunit, inactive mutant	Xenopus laevis
additional information	microinjection of mutant subunits C in Xenopus oocytes, overview	Xenopus laevis
R133A	site-directed mutagenesis of the catalytic subunit, mutant shows decreased interaction with the regulatory RII subunit	Xenopus laevis
R133A/K72H	site-directed mutagenesis of the catalytic subunit, inactive mutant showing decreased interaction with the regulatory RII subunit	Xenopus laevis

Inhibitors

Inhibitors	Comment	Organism	Structure
PKI	recombinant His-tagged rabbit PKA inhibitor protein PKI, binding involves Arg133	Xenopus laevis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Xenopus laevis

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Xenopus laevis	the enzyme regulates the G2/M transition in oocytes of Xenopus laevis, it also blocks progesterone-induced germinal vesicle envelope breakdown GVBD, Cdc-25-dependent dephosphorylation of Tyr15 in Cdc2, and synthesis of MEKK Mos	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Xenopus laevis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
recombinant N-terminally His6-tagged wild-type and mutant catalytic subunits C from Escherichia coli strain BL21(DE3) by nickel affinity chromatography	Xenopus laevis

Source Tissue

Source Tissue	Comment	Organism	Textmining
oocyte	-	Xenopus laevis	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + Kemptide	peptide substrate, activity of catalytic PKA subunit	Xenopus laevis	ADP + phosphorylated Kemptide	-	?
additional information	the enzyme regulates the G2/M transition in oocytes of Xenopus laevis, it also blocks progesterone-induced germinal vesicle envelope breakdown GVBD, Cdc-25-dependent dephosphorylation of Tyr15 in Cdc2, and synthesis of MEKK Mos	Xenopus laevis	?	-	?

Subunits

Subunits	Comment	Organism
More	Arg133 is essential for binding of the catalytic subunit C to the regulatory subunit RII	Xenopus laevis

Synonyms

Synonyms	Comment	Organism
PKA	-	Xenopus laevis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
30	-	assay at	Xenopus laevis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.4	-	assay at	Xenopus laevis

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Xenopus laevis

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Release 2023.1 (January 2023)