Activating Compound | Comment | Organism | Structure |
---|---|---|---|
cAMP | dependent on | Xenopus laevis |
Cloned (Comment) | Organism |
---|---|
expression of N-terminally His6-tagged wild-type and mutant catalytic subunits C in Escherichia coli strain BL21(DE3) | Xenopus laevis |
Protein Variants | Comment | Organism |
---|---|---|
D328A | site-directed mutagenesis of the catalytic subunit, mutant shows decreased interaction with the regulatory RI subunit | Xenopus laevis |
D328A/K72H | site-directed mutagenesis of the catalytic subunit, inactive mutant showing decreased interaction with the regulatory RII subunit | Xenopus laevis |
K72H | site-directed mutagenesis of the catalytic subunit, inactive mutant | Xenopus laevis |
K72R | site-directed mutagenesis of the catalytic subunit, inactive mutant | Xenopus laevis |
additional information | microinjection of mutant subunits C in Xenopus oocytes, overview | Xenopus laevis |
R133A | site-directed mutagenesis of the catalytic subunit, mutant shows decreased interaction with the regulatory RII subunit | Xenopus laevis |
R133A/K72H | site-directed mutagenesis of the catalytic subunit, inactive mutant showing decreased interaction with the regulatory RII subunit | Xenopus laevis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
PKI | recombinant His-tagged rabbit PKA inhibitor protein PKI, binding involves Arg133 | Xenopus laevis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | - |
Xenopus laevis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | Xenopus laevis | the enzyme regulates the G2/M transition in oocytes of Xenopus laevis, it also blocks progesterone-induced germinal vesicle envelope breakdown GVBD, Cdc-25-dependent dephosphorylation of Tyr15 in Cdc2, and synthesis of MEKK Mos | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Xenopus laevis | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant N-terminally His6-tagged wild-type and mutant catalytic subunits C from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Xenopus laevis |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
oocyte | - |
Xenopus laevis | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + Kemptide | peptide substrate, activity of catalytic PKA subunit | Xenopus laevis | ADP + phosphorylated Kemptide | - |
? | |
additional information | the enzyme regulates the G2/M transition in oocytes of Xenopus laevis, it also blocks progesterone-induced germinal vesicle envelope breakdown GVBD, Cdc-25-dependent dephosphorylation of Tyr15 in Cdc2, and synthesis of MEKK Mos | Xenopus laevis | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | Arg133 is essential for binding of the catalytic subunit C to the regulatory subunit RII | Xenopus laevis |
Synonyms | Comment | Organism |
---|---|---|
PKA | - |
Xenopus laevis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Xenopus laevis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
assay at | Xenopus laevis |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Xenopus laevis |