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Literature summary for 2.7.11.11 extracted from
- The effect of cAMP and cGMP on the activity and substrate specificity of protein kinase A from methylotrophic yeast Pichia pastoris (2003), Acta Biochim. Pol., 50, 1111-1118.
Activating Compound
| Activating Compound | Comment | Organism | Structure |
|---|---|---|---|
| 8-bromo-cAMP | activates to same extent as cAMP | Komagataella pastoris |  |
| 8-chloro-cAMP | activates to same extent as cAMP | Komagataella pastoris | |
| cAMP | dependent on, degree of enzyme activation by cAMP or cGMP depends on the substrate | Komagataella pastoris | |
| cGMP | activates, degree of enzyme activation by cAMP or cGMP depends on the substrate | Komagataella pastoris | |
| additional information | no stimulation by N6,2'-O-dibutyryl-cAMP | Komagataella pastoris |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Komagataella pastoris | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 50000 | - |
x * 50000, about, regulatory subunit RII, SDS-PAGE | Komagataella pastoris |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Komagataella pastoris | - |
- |
- |
| Komagataella pastoris JC100 | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the enzyme performs autophosphorylation | Komagataella pastoris |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| by ammonium sulfate fractionation, dialysis, DEAE ion exchange and P11 cellulose chromatography, and hydroxyapatite chromatography, concentration steps on PEG20000 | Komagataella pastoris |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + casein | enzyme activity is preferably activated by cAMP, 25% lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated casein | - |
? | |
| ATP + casein | enzyme activity is preferably activated by cAMP, 25% lower activity with cGMP | Komagataella pastoris JC100 | ADP + phosphorylated casein | - |
? | |
| ATP + histone H2A | enzyme activity is preferably activated by cAMP, 40% lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated histone H2A | - |
? | |
| ATP + histone H2A | enzyme activity is preferably activated by cAMP, 40% lower activity with cGMP | Komagataella pastoris JC100 | ADP + phosphorylated histone H2A | - |
? | |
| ATP + histone H3S | enzyme activity is preferably activated by cAMP, 60% lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated histone H3S | - |
? | |
| ATP + histone H3S | enzyme activity is preferably activated by cAMP, 60% lower activity with cGMP | Komagataella pastoris JC100 | ADP + phosphorylated histone H3S | - |
? | |
| ATP + Kemptide | enzyme activity is preferably activated by cAMP, 80% lower activity with cGMP | Komagataella pastoris | ADP + Kemptide phosphate | - |
? | |
| ATP + maltose binding protein | enzyme activity is preferably activated by cAMP, 35% lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated maltose binding protein | - |
? | |
| ATP + phosvitin | enzyme activity is preferably activated by cAMP, 33% lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated phosvitin | - |
? | |
| ATP + protamine sulfate | enzyme activity is about equally activated by cAMP and cGMP, and derivatives of cAMP | Komagataella pastoris | ADP + phosphoprotamine sulfate | - |
? | |
| ATP + pyruvate kinase | enzyme activity is about equally activated by cAMP and cGMP | Komagataella pastoris | ADP + phosphorylated pyruvate kinase | - |
? | |
| ATP + ribosomal protein S6 | substrate from purified Saccharomyces cerevisiae ribosomes, enzyme activity is preferably activated by cAMP, lower activity with cGMP | Komagataella pastoris | ADP + phosphorylated ribosomal protein S6 | - |
? | |
| ATP + ribosomal protein S6 | substrate from purified Saccharomyces cerevisiae ribosomes, enzyme activity is preferably activated by cAMP, lower activity with cGMP | Komagataella pastoris JC100 | ADP + phosphorylated ribosomal protein S6 | - |
? | |
| additional information | the enzyme performs autophosphorylation, substrate specificity depends on activating agents cAMP or cGMP, overview | Komagataella pastoris | ? | - |
? | |
| additional information | the enzyme performs autophosphorylation, substrate specificity depends on activating agents cAMP or cGMP, overview | Komagataella pastoris JC100 | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 50000, about, regulatory subunit RII, SDS-PAGE | Komagataella pastoris |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| cyclic AMP dependent protein kinase | - |
Komagataella pastoris |
| PKA | - |
Komagataella pastoris |
| protein kinase A | - |
Komagataella pastoris |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Komagataella pastoris |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.5 | - |
assay at | Komagataella pastoris |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Komagataella pastoris | |
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