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Literature summary for 2.7.11.10 extracted from

  • Ha, K.H.; Byun, M.S.; Choi, J.; Jeong, J.; Lee, K.J.; Jue, D.M.
    N-tosyl-L-phenylalanine chloromethyl ketone inhibits NF-kappaB activation by blocking specific cysteine residues of IkappaB kinase beta and p65/RelA (2009), Biochemistry, 48, 7271-7278.
    View publication on PubMed

Application

Application Comment Organism
medicine N-tosyl-L-phenylalanine chloromethyl ketone has been shown to have therapeutic effects in hypoxic-ischemic brain injury and collagen-induced arthritis in experimental animals, without any obvious adverse side effects Homo sapiens

Cloned(Commentary)

Cloned (Comment) Organism
a Flag-tagged IKKbeta expression vector is used Homo sapiens

Protein Variants

Protein Variants Comment Organism
C179A mutant, not susceptible to the inhibitor N-tosyl-L-phenylalanine chloromethyl ketone Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
N-tosyl-L-phenylalanine chloromethyl ketone
-
Homo sapiens

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
700000
-
IKK complex Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [IkappaB protein] Homo sapiens
-
ADP + [IkappaB phosphoprotein]
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
cytoplasmic extracts are prepared Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
HEK-293 cell
-
Homo sapiens
-
HeLa cell
-
Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [IkappaB protein]
-
Homo sapiens ADP + [IkappaB phosphoprotein]
-
?

Subunits

Subunits Comment Organism
oligomer the IkappaB kinase complex is composed of two catalytic subunits, IKKalpha and IKKbeta, or IKK1 and IKK2, and a regulatory subunit, IKKgamma, NEMO or IKKAP1 Homo sapiens

Synonyms

Synonyms Comment Organism
IkappaB kinase beta
-
Homo sapiens
IKKbeta
-
Homo sapiens

Cofactor

Cofactor Comment Organism Structure
ATP
-
Homo sapiens

General Information

General Information Comment Organism
physiological function IKKbeta is shown to play an essential role in the activation of NF-kappaB in response to TNF-alpha and other inflammatory stimuli Homo sapiens