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Literature summary for 2.7.11.10 extracted from

  • Furuya, K.; Ozaki, T.; Hanamoto, T.; Hosoda, M.; Hayashi, S.; Barker, P.A.; Takano, K.; Matsumoto, M.; Nakagawara, A.
    Stabilization of p73 by nuclear IkappaB kinase-alpha mediates cisplatin-induced apoptosis (2007), J. Biol. Chem., 282, 18365-18378.
    View publication on PubMed
Search for more literature for 2.7.11.10

Cloned(Commentary)

Cloned (Comment) Organism
expression of IKKalpha in COS-7 cells, co-expression with p73alpha and His-tagged ubiquitin, analysis of ubiquitinated compounds Mus musculus

Protein Variants

Protein Variants Comment Organism
K44A site-directed mutagenesis, inactive ATP-binding site mutant, the kinase-deficient mutant IKK-alpha fails to stabilize p73 Mus musculus
additional information small interfering RNA-mediated knockdown of endogenous IKK-alpha inhibits the CDDP-mediated accumulation of p73alpha, the kinase-deficient mutant form of IKK-alpha interacts with p73alpha, but fails to stabilize it, CDDP-mediated accumulation of endogenous p73alpha is not detected in mouse embryonic fibroblasts prepared from IKK-alpha-deficient mice, and CDDP sensitivity is significantly decreased compared to wild-type MEFs, overview Mus musculus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol IKKalpha shuttles between the nucleus and cytoplasm in a CRM1-dependent fashion Mus musculus 5829
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nucleus nuclear accumulation of IKKalpha occurs in response to CDDP, IKKalpha shuttles between the nucleus and cytoplasm in a CRM1-dependent fashion Mus musculus 5634
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus IKKalpha enhances p73-mediated transactivation and pro-apoptotic functions in p53-deficient H1299 cells, stabilization of p73, but not of the antagonist p53, by nuclear IKKalpha mediates cisplatin-induced apoptosis, DNA damage-induced accumulation of both p53 and p73alpha is associated with the up-regulation of IKK-alpha and IKK-gamma, a functional interaction might exist between them in DNA damage-mediated apoptotic pathways ?
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 ?

Organism

Organism UniProt Comment Textmining
Mus musculus
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-
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information IKKalpha enhances p73-mediated transactivation and pro-apoptotic functions in p53-deficient H1299 cells, stabilization of p73, but not of the antagonist p53, by nuclear IKKalpha mediates cisplatin-induced apoptosis, DNA damage-induced accumulation of both p53 and p73alpha is associated with the up-regulation of IKK-alpha and IKK-gamma, a functional interaction might exist between them in DNA damage-mediated apoptotic pathways Mus musculus ?
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 ?
additional information IKKalpha interacts with p73 Mus musculus ?
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 ?

Synonyms

Synonyms Comment Organism
IkappaB kinase-alpha
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 Mus musculus
IKKalpha
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 Mus musculus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Mus musculus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
 assay at Mus musculus

Cofactor

Cofactor Comment Organism Structure
ATP the binding involves Lys44 Mus musculus