Cloned (Comment) | Organism |
---|---|
cloning of catalytic subunit alpha of CK2, i.e. ZmCK2alpha-1, and expression as GST-tagged enzyme in Escherichia coli strain BL21(DE3) | Zea mays |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
nucleus | - |
Zea mays | 5634 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Mg2+ | required | Zea mays |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + maize eukaryotic translation initiation factor 5A | Zea mays | - |
ADP + phosphorylated maize eukaryotic translation initiation factor 5A | - |
? | |
ATP + maize eukaryotic translation initiation factor 5A | Zea mays Mona | - |
ADP + phosphorylated maize eukaryotic translation initiation factor 5A | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Zea mays | - |
- |
- |
Zea mays Mona | - |
- |
- |
Purification (Comment) | Organism |
---|---|
native CK2 from seedlings by ammonium sulfate fractionation, cation exchange chromatography, ultrafiltration, dialysis, anion exchange chromatography, again ultrafiltration, and gel filtration | Zea mays |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | - |
Zea mays | - |
seedling | - |
Zea mays | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + maize eukaryotic translation initiation factor 5A | - |
Zea mays | ADP + phosphorylated maize eukaryotic translation initiation factor 5A | - |
? | |
ATP + maize eukaryotic translation initiation factor 5A | phosphorylation at Ser2 by CK2. Zea mays, Arabidopsis thaliana, and Saccharomyces cerevisiae eIF5A wild-type proteins are efficient substrates, while their S2A mutants are not phosphorylated, the S4A mutant is phosphorylated | Zea mays | ADP + phosphorylated maize eukaryotic translation initiation factor 5A | - |
? | |
ATP + maize eukaryotic translation initiation factor 5A | - |
Zea mays Mona | ADP + phosphorylated maize eukaryotic translation initiation factor 5A | - |
? | |
ATP + maize eukaryotic translation initiation factor 5A | phosphorylation at Ser2 by CK2. Zea mays, Arabidopsis thaliana, and Saccharomyces cerevisiae eIF5A wild-type proteins are efficient substrates, while their S2A mutants are not phosphorylated, the S4A mutant is phosphorylated | Zea mays Mona | ADP + phosphorylated maize eukaryotic translation initiation factor 5A | - |
? |
Synonyms | Comment | Organism |
---|---|---|
casein kinase 2 | - |
Zea mays |
CK2 | - |
Zea mays |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
assay at | Zea mays |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | - |
Zea mays |
General Information | Comment | Organism |
---|---|---|
physiological function | phosphorylation of Ser2 plays a role in regulation of nucleocytoplasmic shuttling of eukaryotic translation initiation factor 5A in plant cells | Zea mays |