Protein Variants | Comment | Organism |
---|---|---|
DELTA399-502/T389E | an N-terminal His6 affinity tagged catalytic kinase domain construct of the alphaII isoform of S6K1 lacking the autoinhibitory domain (AID) (His6-S6K1alphaII(DELTA AID)-T389E), is generated in its most highly active form (250 nmol/min/mg) by baculovirus-mediated expression and purification from Sf9 insect cells that are coinfected with recombinant PDK1. kcat (1/sec): 0.19 (RRRLSSLRA) in vivo assay, 0.057 (RRRLSSLRA) in vitro assay, 0.19 (ATP) in vivo assay, 0.057 (ATP) in vitro assay. Km (mM): 3.6 (RRRLSSLRA) in vivo assay, 3.7 (RRRLSSLRA) in vitro assay, 5.4 (ATP) in vivo assay, 4.8 (ATP) in vitro assay | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0036 | - |
RRRLSSLRA | in vivo PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E) | Homo sapiens | |
0.0037 | - |
RRRLSSLRA | in vitro PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E) | Homo sapiens | |
0.0048 | - |
ATP | in vitro PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E) | Homo sapiens | |
0.0054 | - |
ATP | in vivo PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E) | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
an N-terminal His6 affinity tagged catalytic kinase domain construct of the alphaII isoform of S6K1 lacking the autoinhibitory domain (AID) (His6-S6K1alphaII(DELTA AID)-T389E), is expressed in Escherichia coli and purified by His6 affinity co-purification and by MonoQ anion exchange chromatography at pH 7.3 | Homo sapiens |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.25 | - |
specific activity of an N-terminal His6 affinity tagged catalytic kinase domain construct of the alphaII isoform of S6K1 lacking the autoinhibitory domain (AID) (DELTA399-502/T389E) | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + RRRLSSLRA | enzyme activities are determined by measuring initial velocities for His6-S6K1aII(DELTA AID)-T389E catalyzed phosphorylation of a commercially available S6K/RSK model peptide substrate 1 (S6K1-Tide, RRRLSSLRA) | Homo sapiens | ADP + RRRLSpSLRA | - |
? |
Synonyms | Comment | Organism |
---|---|---|
S6K1alphaII | - |
Homo sapiens |
serine-threonine kinase | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
assay at | Homo sapiens |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.057 | - |
ATP | in vitro PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E) | Homo sapiens | |
0.057 | - |
RRRLSSLRA | in vitro PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E) | Homo sapiens | |
0.19 | - |
ATP | in vivo PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E) | Homo sapiens | |
0.19 | - |
RRRLSSLRA | in vivo PDK1-activated mutant DELTA399-502/T389E (His6-S6K1aII(DELTA AID)-T389E) | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
assay at | Homo sapiens |