Literature summary for 2.7.11.1 extracted from

Gopalaswamy, R.; Narayanan, S.; Jacobs, W.R.; Av-Gay, Y.
Mycobacterium smegmatis biofilm formation and sliding motility are affected by the serine/threonine protein kinase PknF (2008), FEMS Microbiol. Lett., 278, 121-127.

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Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli as a His-tagged fusion protein	Mycolicibacterium smegmatis

Protein Variants

Protein Variants	Comment	Organism
K41M	mutated residue locates in subdomain II of STPKs, is highly conserved and results in loss of kinase activity	Mycolicibacterium smegmatis
additional information	the Ms-pknF gene is overexpressed in Mycobacterium smegmatis mc2155 under the control of an inducible acetamidase promoter. The overexpressing strain displays altered colony morphology, defective slidingmotility and biofilm formation. These data indicate a role for PknF in biofilm formation, possibly associated with alterations in glycopeptidolipid composition	Mycolicibacterium smegmatis

Molecular Weight [Da]

Molecular Weight [Da]	Molecular Weight Maximum [Da]	Comment	Organism
56000	-	SDS-PAGE	Mycolicibacterium smegmatis

Organism

Organism	UniProt	Comment	Textmining
Mycolicibacterium smegmatis	-	-	-

Synonyms

Synonyms	Comment	Organism
Ms-pknF	-	Mycolicibacterium smegmatis
serine-threonine kinase PknF	-	Mycolicibacterium smegmatis

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Release 2023.1 (January 2023)