Literature summary for 2.7.11.1 extracted from

LaRonde-LeBlanc, N.; Wlodawer, A.
Crystal structure of A. fulgidus Rio2 defines a new family of serine protein kinases (2004), Structure, 12, 1585-1594.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of Rio2 in Escherichia coli	Archaeoglobus fulgidus

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant wild-type and selenomethionine-labeled Rio2, hanging drop vapour diffusion method, equal volumes of protein solution, containing 5-12% PEG 900, and 0.1 M phosphate citrate buffer, pH 3.6-4.1, and reservoir solution, versus 1 ml reservoir solution, purified recombinant Rio2 complexed with ATP using 0.1 M Tris, pH 7.5, 20 mM ATP, 20 mM MgCl2, 20 mM adenosine 5'-(beta,gamma-imino)triphosphate, and 20% ethylene glycol, 20°C, 2-4 days, X-ray diffraction structure determination and analysis at 2.0 A resolution	Archaeoglobus fulgidus

Crystallization (Comment)

Organism

purified recombinant wild-type and selenomethionine-labeled Rio2, hanging drop vapour diffusion method, equal volumes of protein solution, containing 5-12% PEG 900, and 0.1 M phosphate citrate buffer, pH 3.6-4.1, and reservoir solution, versus 1 ml reservoir solution, purified recombinant Rio2 complexed with ATP using 0.1 M Tris, pH 7.5, 20 mM ATP, 20 mM MgCl2, 20 mM adenosine 5'-(beta,gamma-imino)triphosphate, and 20% ethylene glycol, 20°C, 2-4 days, X-ray diffraction structure determination and analysis at 2.0 A resolution

Archaeoglobus fulgidus

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mg2+	-	Archaeoglobus fulgidus

Natural Substrates/ Products (Substrates)

Natural Substrates	Organism	Comment (Nat. Sub.)	Natural Products	Comment (Nat. Pro.)	Rev.	Reac.
additional information	Archaeoglobus fulgidus	kinase Rio2 is required for rRNA cleavage in 40S ribosomal subunit maturation	?	-	?

Organism

Organism	UniProt	Comment	Textmining
Archaeoglobus fulgidus	O30245	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	Rio2 performs autophosphorylation	Archaeoglobus fulgidus

Purification (Commentary)

Purification (Comment)	Organism
recombinant Rio2 from Escherichia coli by adsorption chromatography and gel filtration	Archaeoglobus fulgidus

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.
ATP + alpha-casein	dephosphorylated alpha-casein	Archaeoglobus fulgidus	ADP + phosphorylated alpha-casein	-	?
ATP + histone	-	Archaeoglobus fulgidus	ADP + phosphorylated histone	-	?
ATP + myelin basic protein	-	Archaeoglobus fulgidus	ADP + phosphorylated myelin basic protein	-	?
additional information	kinase Rio2 is required for rRNA cleavage in 40S ribosomal subunit maturation	Archaeoglobus fulgidus	?	-	?
additional information	Rio2 performs autophosphorylation	Archaeoglobus fulgidus	?	-	?

Subunits

Subunits	Comment	Organism
More	the kinase domain is tightly connected to a wHTH domain	Archaeoglobus fulgidus

Synonyms

Synonyms	Comment	Organism
More	the enzyme belongs to the Rio family, Rio2 subfamily, of serine/threonine protein kinases	Archaeoglobus fulgidus
Rio2	-	Archaeoglobus fulgidus

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
37	-	assay at	Archaeoglobus fulgidus

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7.5	-	assay at	Archaeoglobus fulgidus

Cofactor

Cofactor	Comment	Organism	Structure
ATP	binding structure involving residues K168, S53, and D166, overview	Archaeoglobus fulgidus