Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 2.7.11.1 extracted from

  • Dissanayake, K.; Castillo, C.; Takasaki, T.; Nakanishi, T.; Norioka, N.; Norioka, S.
    Molecular cloning, functional expression and characterization of two serine/threonine-specific protein kinases from Nicotiana tabacum pollen (2004), Sex. Plant Reprod., 17, 165-175.
    View publication on PubMed
Search for more literature for 2.7.11.1

Cloned(Commentary)

Cloned (Comment) Organism
DNA and amino acid sequence determination and analysis of protein kinase PK1, expression as GST-fusion protein in Escherichia coli strain BL21 Nicotiana tabacum
DNA and amino acid sequence determination and analysis of protein kinase PK2, expression as GST-fusion protein in Escherichia coli strain BL21 Nicotiana tabacum

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Nicotiana tabacum 5829
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Nicotiana tabacum
Mn2+
-
 Nicotiana tabacum

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
39200
-
 x * 39200, PK1, calculated from amino acid sequence Nicotiana tabacum
39500
-
 x * 39500, PK2, calculated from amino acid sequence Nicotiana tabacum

Organism

Organism UniProt Comment Textmining
Nicotiana tabacum Q709M0 cv. Petit Havana
-
Nicotiana tabacum Q709M1 cv. Petit Havana
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein autophosphorylation of PK1 Nicotiana tabacum
phosphoprotein autophosphorylation of PK2 Nicotiana tabacum

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-fusion PK1 from Escherichia coli strain BL21 by glutathione affinity chromatography, the GST-tag is cleaved off Nicotiana tabacum
recombinant GST-fusion PK2 from Escherichia coli strain BL21 by glutathione affinity chromatography, the GST-tag is cleaved off Nicotiana tabacum

Source Tissue

Source Tissue Comment Organism Textmining
additional information developmental expression, overview Nicotiana tabacum
-
additional information PK1 is not expressed in other tissues, developmental expression in pollen, overview Nicotiana tabacum
-
petal
-
 Nicotiana tabacum
-
pistil
-
 Nicotiana tabacum
-
pollen
-
 Nicotiana tabacum
-
pollen tube
-
 Nicotiana tabacum
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information PK1 performs autophosphorylation Nicotiana tabacum ?
-
 ?
additional information PK2 performs autophosphorylation Nicotiana tabacum ?
-
 ?

Subunits

Subunits Comment Organism
? x * 39200, PK1, calculated from amino acid sequence Nicotiana tabacum
? x * 39500, PK2, calculated from amino acid sequence Nicotiana tabacum

Synonyms

Synonyms Comment Organism
PK1
-
 Nicotiana tabacum
PK2
-
 Nicotiana tabacum
serine/threonine-specific protein kinase
-
 Nicotiana tabacum

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Nicotiana tabacum

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7.5
-
 assay at Nicotiana tabacum

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Nicotiana tabacum