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Literature summary for 2.7.11.1 extracted from
- Blue light-regulated molecular switch of Ser/Thr kinase in phototropin (2005), Proc. Natl. Acad. Sci. USA, 102, 13337-13342.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of GST-fusion LOV2 and kinase domain LV2-KD of phototropin in Escherichia coli | Arabidopsis thaliana |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| additional information | the chromophoric domain light-oxygen-voltage-sensing 2 LOV2 of phototropin acts as an intramolecular inhibitor to substrate protein phosphorylation | Arabidopsis thaliana |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| chloroplast | - |
Arabidopsis thaliana | 9507 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Arabidopsis thaliana |  |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| additional information | Arabidopsis thaliana | photoregulation of phototropin, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Arabidopsis thaliana | - |
- |
- |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | phototropin performs autophosphorylation | Arabidopsis thaliana |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant GST-fusion LOV2 and kinase domain LV2-KD of phototropin from Escherichia coli by glutathione affinity chromatography | Arabidopsis thaliana |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + casein | the chromophoric domain light-oxygen-voltage-sensing 2 LOV2 of phototropin acts as a blue light-regulated molecular switch | Arabidopsis thaliana | ADP + phosphorylated casein | - |
? | |
| additional information | photoregulation of phototropin, overview | Arabidopsis thaliana | ? | - |
? | |
| additional information | enzyme assay under dim red light conditions, phototropin performs autophosphorylation | Arabidopsis thaliana | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| More | phototropin has 2 chromophoric domains, the light-oxygen-voltage-sensing 1 and 2, i.e. LOV1 and LOV2, in the N-terminal half, and a Ser/Thr kinase motif in the C-terminal half | Arabidopsis thaliana |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| Phototropin | - |
Arabidopsis thaliana |
| Ser/Thr kinase | - |
Arabidopsis thaliana |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Arabidopsis thaliana |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.8 | - |
assay at | Arabidopsis thaliana |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Arabidopsis thaliana | |
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