Literature summary for 2.7.11.1 extracted from

Ortiz-Lombardia, M.; Pompeo, F.; Boitel, B.; Alzari, P.M.
Crystal structure of the catalytic domain of the PknB serine/threonine kinase from Mycobacterium tuberculosis (2003), J. Biol. Chem., 278, 13094-13100.

Cloned(Commentary)

Cloned (Comment)	Organism
expression of N-terminal catalytic domain of PknB, comprising residues 1-331, and of a construct comprising residues 1-279 in Escherichia coli BL21(DE3) as His-tagged proteins	Mycobacterium tuberculosis

Crystallization (Commentary)

Crystallization (Comment)	Organism
purified recombinant His-tagged N-terminal catalytic domain of PknB, residues 1-331, hanging drop vapour diffusion method, 0.001 ml protein solution containing 5 mg/ml protein mixed with equal volume of 0.1 M HEPES, pH 7.5, 30 mM MgCl2, 0.15 mM inhibitor AMP-PNP, 27% PEG 400, and 4% 1,3-butanediol, 19°C, versus 1 ml of a solution containing 0.1 M HEPES, pH 7.5, 30 mM MgCl2, and 27% PEG 400, 2-3 weeks, X-ray diffraction structure determination and analysis at 2.2 A resolution	Mycobacterium tuberculosis

Crystallization (Comment)

Organism

purified recombinant His-tagged N-terminal catalytic domain of PknB, residues 1-331, hanging drop vapour diffusion method, 0.001 ml protein solution containing 5 mg/ml protein mixed with equal volume of 0.1 M HEPES, pH 7.5, 30 mM MgCl2, 0.15 mM inhibitor AMP-PNP, 27% PEG 400, and 4% 1,3-butanediol, 19°C, versus 1 ml of a solution containing 0.1 M HEPES, pH 7.5, 30 mM MgCl2, and 27% PEG 400, 2-3 weeks, X-ray diffraction structure determination and analysis at 2.2 A resolution

Mycobacterium tuberculosis

Inhibitors

Inhibitors	Comment	Organism	Structure
AMP-PNP	ATP analogue adenosine 5'-(beta,gamma-imino)triphosphate	Mycobacterium tuberculosis

Metals/Ions

Metals/Ions	Comment	Organism	Structure
Mn2+	-	Mycobacterium tuberculosis

Organism

Organism	UniProt	Comment	Textmining
Mycobacterium tuberculosis	-	-	-

Posttranslational Modification

Posttranslational Modification	Comment	Organism
phosphoprotein	PknB is autophosphorylated	Mycobacterium tuberculosis

Purification (Commentary)

Purification (Comment)	Organism
recombinant His-tagged N-terminal catalytic domain of PknB and His-tagged residues 1-279 construct from Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration	Mycobacterium tuberculosis

Reaction

Reaction	Comment	Organism	Reaction ID
ATP + a [protein]-(L-serine/L-threonine) = ADP + a [protein]-(L-serine/L-threonine) phosphate	active site structure	Mycobacterium tuberculosis	a

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + myelic basic protein	phosphorylation of serine and threonine residues by PknB	Mycobacterium tuberculosis	ADP + myelic basic phosphoprotein	-	?
additional information	PknB performs autophosphorylation	Mycobacterium tuberculosis	?	-	?

Synonyms

Synonyms	Comment	Organism
PknB	-	Mycobacterium tuberculosis
serine/threonine kinase	-	Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C]	Temperature Optimum Maximum [°C]	Comment	Organism
22	-	assay at room temperature	Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
7	-	assay at	Mycobacterium tuberculosis

Cofactor

Cofactor	Comment	Organism	Structure
ATP	-	Mycobacterium tuberculosis