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Literature summary for 2.7.11.1 extracted from

  • Duran, R.; Villarino, A.; Bellinzoni, M.; Wehenkel, A.; Fernandez, P.; Boitel, B.; Cole, S.T.; Alzari, P.M.; Cervenansky, C.
    Conserved autophosphorylation pattern in activation loops and juxtamembrane regions of Mycobacterium tuberculosis Ser/Thr protein kinases (2005), Biochem. Biophys. Res. Commun., 333, 858-867.
    View publication on PubMed

Activating Compound

Activating Compound Comment Organism Structure
additional information autophosphorylation patterns for activation of PknB, PknD, PknE, and PknF, e.g. PknB phosphorylation at Thr171 and Thr173 of the activation loop and at Thr294 of the juxtamembrane domain are necessary for activity Mycobacterium tuberculosis

Cloned(Commentary)

Cloned (Comment) Organism
expression of His-tagged PknB, PknD, PknE, and PknF in Escherichia coli strain BL21(DE3) Mycobacterium tuberculosis

Localization

Localization Comment Organism GeneOntology No. Textmining
membrane
-
Mycobacterium tuberculosis 16020
-
additional information PknB, PknD, PknE, and PknF possess an N-terminal kinase domain, a juxtamembrane domain of varying length, and an activation loop Mycobacterium tuberculosis
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mn2+
-
Mycobacterium tuberculosis

Organism

Organism UniProt Comment Textmining
Mycobacterium tuberculosis
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein PknB, PknD, PknE, and PknF are autophosphorylated at multiple sites, phosphorylation patterns, overview, the enzymes are dephosphorylated by the cognate mycobacterial phosphatase PstP Mycobacterium tuberculosis

Purification (Commentary)

Purification (Comment) Organism
recombinant His-tagged PknB, PknD, PknE, and PknF rom Escherichia coli strain BL21(DE3) by nickel affinity chromatography and gel filtration Mycobacterium tuberculosis

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + myelin basic protein PknB, PknD, PknE, and PknF Mycobacterium tuberculosis ADP + phosphorylated myelin basic protein
-
?
additional information PknB, PknD, PknE, and PknF perform autophosphorylation Mycobacterium tuberculosis ?
-
?

Subunits

Subunits Comment Organism
More PknB, PknD, PknE, and PknF possess an N-terminal kinase domain, a juxtamembrane domain of varying length, and an activation loop Mycobacterium tuberculosis

Synonyms

Synonyms Comment Organism
PknB
-
Mycobacterium tuberculosis
PknD
-
Mycobacterium tuberculosis
PknE
-
Mycobacterium tuberculosis
PknF
-
Mycobacterium tuberculosis
Ser/Thr protein kinase
-
Mycobacterium tuberculosis

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
assay at Mycobacterium tuberculosis

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
assay at Mycobacterium tuberculosis

Cofactor

Cofactor Comment Organism Structure
ATP
-
Mycobacterium tuberculosis