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Literature summary for 2.7.11.1 extracted from

  • Amick, G.D.; Reddy, S.A.G.; Damuni, Z.
    Purification and properties of a protamine kinase from bovine kidney microsomes (1992), Arch. Biochem. Biophys., 297, 80-85.
    View publication on PubMed
Search for more literature for 2.7.11.1

General Stability

General Stability Organism
Triton X-100 stabilizes during freeze-thawing procedures Bos taurus

Inhibitors

Inhibitors Comment Organism Structure
Ca2+ not Bos taurus
Catalytic subunit of protein phosphatase 2A
-
 Bos taurus
KCl not Bos taurus
additional information EGTA, calmodulin; heparin, Ca2+/calmodulin, phosphatidylserine and/or diolein (in the absence or presence of Ca2+) Bos taurus
NaCl not Bos taurus
Protein phosphatase 2A 2A1 and 2; ATP; okadaic acid, microcystin-LR; phosphate or NaF prevents; specific protamine kinase inhibitors Bos taurus

Localization

Localization Comment Organism GeneOntology No. Textmining
cytosol
-
 Bos taurus 5829
-
microsome microsomal kinase is a form of the cytosolic kinase Bos taurus
-
-
soluble
-
 Bos taurus
-
-

Metals/Ions

Metals/Ions Comment Organism Structure
Mg2+
-
 Bos taurus
Mn2+ can replace Mg2+ with 20% efficiency Bos taurus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
45000
-
 gel filtration Bos taurus

Organism

Organism UniProt Comment Textmining
Bos taurus
-
-
-

Purification (Commentary)

Purification (Comment) Organism
microsomal Bos taurus

Source Tissue

Source Tissue Comment Organism Textmining
kidney
-
 Bos taurus
-

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
7.53
-
-
 Bos taurus

Storage Stability

Storage Stability Organism
-70°C, in 0.025 M Tris-HCl, pH 7.3, 10% glycerol, 1 mM EDTA, 1 mM benzamidine, 0.1 mM PMSF, 14 mM 2-mercaptoethanol, 0.1% Triton X-100, 12 months Bos taurus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + casein phosphorylated at 5% the rate of protamine, cytosolic kinase Bos taurus ?
-
 ?
ATP + histone H1 not microsomal kinases Bos taurus ?
-
 ?
ATP + histone IIB phosphorylated at 8% the rate of protamine, cytosolic kinase Bos taurus ?
-
 ?
ATP + protamine no substrates are GTP Bos taurus ADP + O-phosphoprotamine
-
 ?
ATP + several 40S ribosomal polypeptides e.g. S6, 2.5 mol phosphate per mol S6 Bos taurus ?
-
 ?
ATP + synthetic peptide Arg-Arg-Leu-Ser-Ser-Leu-Arg-Ala Bos taurus ?
-
 ?

Subunits

Subunits Comment Organism
monomer 1 * 45000, SDS-PAGE Bos taurus

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
 assay at Bos taurus

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
6 9
-
 Bos taurus