Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli under a T7 promoter | Myxococcus xanthus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
membrane | Pkn2 is a transmembrane protein with the kinase domain in the cytoplasm and the 207-residue carboxy-terminal domain outside the cytoplasmic membrane | Myxococcus xanthus | 16020 | - |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | Myxococcus xanthus | the enzyme blocks the secretion of beta-lactamase by phosphorylation | ADP + a phosphoprotein | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Myxococcus xanthus | P54736 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
phosphoprotein | phosphorylated at both Ser and Thr residues | Myxococcus xanthus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + a protein | the enzyme blocks the secretion of beta-lactamase by phosphorylation | Myxococcus xanthus | ADP + a phosphoprotein | - |
? | |
ATP + TEM-beta-lactamase | the enzyme is phosphorylated only at Thr residues, shifting its apparent molecular mass from 29000 Da to 44000 Da. The phosphorylated beta-lactamase is unable to be secreted into the periplasmic space and localized in the cytoplasmic and membrane fractions | Myxococcus xanthus | ADP + phosphorylated TEM-beta-lactamase | - |
? |
Synonyms | Comment | Organism |
---|---|---|
serine/threonine-protein kinase pkn2 | - |
Myxococcus xanthus |