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Literature summary for 2.7.10.2 extracted from

  • Lee, D.C.; Zheng, J.; She, Y.M.; Jia, Z.
    Structure of Escherichia coli tyrosine kinase Etk reveals a novel activation mechanism (2008), EMBO J., 27, 1758-1766.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.10.2

Activating Compound

Activating Compound Comment Organism Structure
additional information protein kinase activation through an intra-molecular autophosphorylation process Escherichia coli

Cloned(Commentary)

Cloned (Comment) Organism
the Etk C-terminal kinase domain, 451-726, and Y574A, R614A mutants are cloned into pET vectors for expression in Escherichia coli BL21DE3 cells Escherichia coli

Crystallization (Commentary)

Crystallization (Comment) Organism
the C-terminal kinase domain of Escherichia coli Tyr kinase Etk is solved at 2.5 A resolution Escherichia coli

Protein Variants

Protein Variants Comment Organism
R614A mutant, used in autokinase activity assay Escherichia coli
R614K mutant, used in autokinase activity assay Escherichia coli
Y574A mutant, used in autokinase activity assay Escherichia coli
Y574E mutant, used in autokinase activity assay Escherichia coli
Y574F mutant, used in autokinase activity assay Escherichia coli
Y574G mutant, used in autokinase activity assay Escherichia coli
Y574N mutant, used in autokinase activity assay Escherichia coli

Localization

Localization Comment Organism GeneOntology No. Textmining
inner membrane
-
 Escherichia coli
-
-

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [protein]-L-tyrosine Escherichia coli
-
 ADP + [protein]-L-tyrosine phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
Escherichia coli P58764
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein
-
 Escherichia coli

Purification (Commentary)

Purification (Comment) Organism
by nickel nitrilotriacetate agarose affinity chromatography and gel filtration Escherichia coli

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
-
 wild type activity 100%, mutant Y574A 98.4%, mutant Y574E 100%, mutant Y574F 12.1%, mutant Y574G 86.7%, mutant Y574N 26.9%, mutant R614A 14.6%, mutant R614K 61.8% Escherichia coli

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [protein]-L-tyrosine
-
 Escherichia coli ADP + [protein]-L-tyrosine phosphate
-
 ?

Synonyms

Synonyms Comment Organism
tyrosine kinase Etk
-
 Escherichia coli

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
22
-
 kinase activity assay at room temperature Escherichia coli

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8.5
-
 kinase activity assay Escherichia coli

Cofactor

Cofactor Comment Organism Structure
ATP
-
 Escherichia coli