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Literature summary for 2.7.10.2 extracted from

  • Brdicka, T.; Kadlecek, T.A.; Roose, J.P.; Pastuszak, A.W.; Weiss, A.
    Intramolecular regulatory switch in ZAP-70: analogy with receptor tyrosine kinases (2005), Mol. Cell. Biol., 25, 4924-4933.
    View publication on PubMedView publication on EuropePMC
Search for more literature for 2.7.10.2

Cloned(Commentary)

Cloned (Comment) Organism
ZAP-70 DNA sequence determination and analysis, stable expression of Syk or ZAP-70 in enzyme-deficient Jurkat-cell-derived P116 cells, transient expression in COS or T293 cells Homo sapiens

Protein Variants

Protein Variants Comment Organism
Y315A site-directed mutagenesis, activity is similar to the wild-type ZAP-70 Homo sapiens
Y315F site-directed mutagenesis, inactive ZAP-70 mutant Homo sapiens
Y319A site-directed mutagenesis, activity is similar to the wild-type ZAP-70 Homo sapiens
Y319F site-directed mutagenesis, inactive ZAP-70 mutant Homo sapiens

Inhibitors

Inhibitors Comment Organism Structure
interdomain B connects the enzyme's catalytic kinase domain with its SH2 domain, is responsible for regulatory autoinhibition of ZAP-70 involving Tyr315 and Tyr319, deletion of the interdomain B preserves enzyme function, the regulation mechanism is similar to receptor protein tyrosine kinase EphB2, EC 2.7.10.1, overview Homo sapiens

Localization

Localization Comment Organism GeneOntology No. Textmining
cytoplasm
-
 Homo sapiens 5737
-

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
70000
-
 x * 70000, about, ZAP-70, SDS-PAGE Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + [transmembrane adaptor protein LAT]-L-tyrosine Homo sapiens substrate of ZAP-70 ADP + [transmembrane adaptor protein LAT]-L-tyrosine phosphate
-
 ?
ATP + [ZAP-70]-L-tyrosine Homo sapiens substrate of Lck, Fyn, and c-Abl, leads to ZAP-70 activation, mechanism ADP + [ZAP-70]-L-tyrosine phosphate
-
 ?
additional information Homo sapiens the Syk family tyrosine kinase ZAP-70 is required to couple the activated T-cell antigen receptor TCR to downstream signaling pathways, binding of TCR via its SH2 domains, regulation mechanism, overview ?
-
 ?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
phosphoprotein ZAP-70 is phosphorylated by Lck, Fyn, and c-Abl protein tyrosine kinases at Y319, and possibly at Y319, leads to ZAP-70 activation via phosphorylation at Y493 of the activation loop, mechanism Homo sapiens

Source Tissue

Source Tissue Comment Organism Textmining
JURKAT cell
-
 Homo sapiens
-
T-cell
-
 Homo sapiens
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + [transmembrane adaptor protein LAT]-L-tyrosine substrate of ZAP-70 Homo sapiens ADP + [transmembrane adaptor protein LAT]-L-tyrosine phosphate
-
 ?
ATP + [ZAP-70]-L-tyrosine substrate of Lck, Fyn, and c-Abl, leads to ZAP-70 activation, mechanism Homo sapiens ADP + [ZAP-70]-L-tyrosine phosphate
-
 ?
ATP + [ZAP-70]-L-tyrosine substrate of Lck, Fyn, and c-Abl Homo sapiens ADP + [ZAP-70]-L-tyrosine phosphate
-
 ?
additional information the Syk family tyrosine kinase ZAP-70 is required to couple the activated T-cell antigen receptor TCR to downstream signaling pathways, binding of TCR via its SH2 domains, regulation mechanism, overview Homo sapiens ?
-
 ?

Subunits

Subunits Comment Organism
? x * 70000, about, ZAP-70, SDS-PAGE Homo sapiens

Synonyms

Synonyms Comment Organism
c-ABL
-
 Homo sapiens
Fyn
-
 Homo sapiens
Lck
-
 Homo sapiens
More ZAP-70 belongs to the Syk protein tyrosine kinase family Homo sapiens
Protein tyrosine kinase
-
 Homo sapiens
PTK
-
 Homo sapiens
Syk
-
 Homo sapiens
ZAP-70
-
 Homo sapiens