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Literature summary for 2.7.10.2 extracted from
- Role of Yes kinase during early zebrafish development (2005), Dev. Biol., 277, 129-141.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression of c-Yes domains U and SH3 as GST-fusion proteins in Escherichia coli strain DH5alpha | Danio rerio |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| 4-amino-5-(4-chlorophenyl)-7-(tert-butyl)pyrazolo[3,4-d] pyrimidine | i.e. pp2, inhibits Src family PTKs, inhibition of zebrafish egg/embryo development by Yes kinase inhibition | Danio rerio |  |
| 4-amino-7-phenylpyrazolo[3,4-d] pyrimidine | i.e. pp3, inhibits Src family PTKs, slight inhibition of zebrafish egg/embryo development by Yes kinase inhibition | Danio rerio | |
Localization
| Localization | Comment | Organism | GeneOntology No. | Textmining |
|---|---|---|---|---|
| plasma membrane | - |
Danio rerio | 5886 | - |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Danio rerio | |
| Mn2+ | - |
Danio rerio | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 62000 | - |
x * 62000, recombinant Yes-kinase, SDS-PAGE | Danio rerio |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [FAK]-L-tyrosine | Danio rerio | activation of FAK during epiboly and gastrulation by dynamic phosphorylation at Tyr861 | ADP + [FAK]-L-tyrosine phosphate | - |
? | |
| additional information | Danio rerio | Yes kinase is required during development, overview | ? | - |
? | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Danio rerio | A1A5H8 | zebrafish | - |
Posttranslational Modification
| Posttranslational Modification | Comment | Organism |
|---|---|---|
| phosphoprotein | the Yes kinase performs autophosphorylation | Danio rerio |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| recombinant c-Yes GST-fusion U and SH3 domains from Escherichia coli strain DH5alpha by glutathione affinity chromatography | Danio rerio |
Source Tissue
| Source Tissue | Comment | Organism | Textmining |
|---|---|---|---|
| egg | - |
Danio rerio | - |
| embryo | - |
Danio rerio | - |
| additional information | enzyme is active at all stages of egg and embryo development | Danio rerio | - |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [FAK]-L-tyrosine | - |
Danio rerio | ADP + [FAK]-L-tyrosine phosphate | - |
? | |
| ATP + [FAK]-L-tyrosine | activation of FAK during epiboly and gastrulation by dynamic phosphorylation at Tyr861 | Danio rerio | ADP + [FAK]-L-tyrosine phosphate | - |
? | |
| additional information | Yes kinase is required during development, overview | Danio rerio | ? | - |
? | |
| additional information | the Yes kinase performs autophosphorylation | Danio rerio | ? | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| ? | x * 62000, recombinant Yes-kinase, SDS-PAGE | Danio rerio |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| More | Yes kinase is a Src-family tyrosine kinase | Danio rerio |
| Yes kinase | - |
Danio rerio |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 25 | - |
assay at | Danio rerio |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Danio rerio |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Danio rerio | |
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