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Literature summary for 2.7.10.2 extracted from
- Western blot analysis of Src kinase assays using peptide substrates ligated to a carrier protein (2004), Biotechniques, 36; 976-978, 980-971.
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Homo sapiens |  |
| Mn2+ | - |
Homo sapiens | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Homo sapiens | - |
- |
- |
Specific Activity [micromol/min/mg]
| Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
|---|---|---|---|
| additional information | - |
assay development using carrier-protein-linked peptides substrate and phosphotyrosine-based Western Blot analysis for detection and quantification, overview | Homo sapiens |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + [carrier protein-intein-CAEEEIYGEFEA]-L-tyrosine | substrate is synthesized by fusing a 27 kDa carrier protein to intein which is linked to the peptide CAEEEIYGEFEA derived from human cyclin-dependent kinase harboring a phosphorylation site for Src kinase | Homo sapiens | ADP + [carrier protein-intein-CAEEEIYGEFEA]-L-tyrosine phosphate | - |
? | |
| ATP + [carrier protein-intein-CIGEGKYGVVYK]-L-tyrosine | substrate is synthesized by fusing a 27 kDa carrier protein to intein which is linked to the peptide CIGEGKYGVVYK derived from human cyclin-dependent kinase harboring a phosphorylation site for Src kinase | Homo sapiens | ADP + [carrier protein-intein-CIGEGKYGVVYK]-L-tyrosine phosphate | - |
? | |
| ATP + [carrier protein-intein-CIGEGTFGVVYK]-L-tyrosine | substrate is synthesized by fusing a 27 kDa carrier protein to intein which is linked to the peptide CIGEGTFGVVYK derived from human cyclin-dependent kinase harboring a phosphorylation site for Src kinase | Homo sapiens | ADP + [carrier protein-intein-CIGEGTFGVVYK]-L-tyrosine phosphate | - |
? | |
| ATP + [carrier protein-intein-CIGEGTpYGVVYK]-L-tyrosine | substrate is synthesized by fusing a 27 kDa carrier protein to intein which is linked to the peptide CIGEGTpYGVVYK derived from human cyclin-dependent kinase harboring a phosphorylation site for Src kinase | Homo sapiens | ADP + [carrier protein-intein-CIGEGTpYGVVYK]-L-tyrosine phosphate | - |
? | |
| ATP + [carrier protein-intein-CIGEGTYFVVYK]-L-tyrosine | substrate is synthesized by fusing a 27 kDa carrier protein to intein which is linked to the peptide CIGEGTYFVVYK derived from human cyclin-dependent kinase harboring a phosphorylation site for Src kinase | Homo sapiens | ADP + [carrier protein-intein-CIGEGTYFVVYK]-L-tyrosine phosphate | - |
? | |
| ATP + [carrier protein-intein-CIGEGTYGVEYK]-L-tyrosine | substrate is synthesized by fusing a 27 kDa carrier protein to intein which is linked to the peptide CIGEGTYGVEYK derived from human cyclin-dependent kinase harboring a phosphorylation site for Src kinase | Homo sapiens | ADP + [carrier protein-intein-CIGEGTYGVEYK]-L-tyrosine phosphate | - |
? | |
| ATP + [carrier protein-intein-CIGEGTYGVVFK]-L-tyrosine | substrate is synthesized by fusing a 27 kDa carrier protein to intein which is linked to the peptide CIGEGTYGVVFK derived from human cyclin-dependent kinase harboring a phosphorylation site for Src kinase | Homo sapiens | ADP + [carrier protein-intein-CIGEGTYGVVFK]-L-tyrosine phosphate | - |
? | |
| ATP + [carrier protein-intein-CIGEGTYGVVYK]-L-tyrosine | substrate is synthesized by fusing a 27 kDa carrier protein to intein which is linked to the peptide CIGEGTYGVVYK derived from human cyclin-dependent kinase harboring a phosphorylation site for Src kinase | Homo sapiens | ADP + [carrier protein-intein-CIGEGTYGVVYK]-L-tyrosine phosphate | - |
? | |
| ATP + [carrier protein-intein-CIGKGTYGVVYK]-L-tyrosine | substrate is synthesized by fusing a 27 kDa carrier protein to intein which is linked to the peptide CIGKGTYGVVYK derived from human cyclin-dependent kinase harboring a phosphorylation site for Src kinase | Homo sapiens | ADP + [carrier protein-intein-CIGKGTYGVVYK]-L-tyrosine phosphate | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| c-Src protein tyrosine kinase | - |
Homo sapiens |
| Src kinase | - |
Homo sapiens |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 30 | - |
assay at | Homo sapiens |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 7.2 | - |
assay at | Homo sapiens |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Homo sapiens | |
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