Activating Compound | Comment | Organism | Structure |
---|---|---|---|
5-aza-2'-deoxycytidine | demethylation by 5-aza-2'-deoxycytidine upregulates Axl and Sp3 expression in low-Axl-expressing Colo206f/WiDr cells, but not in high-Axl-expressing Rko cells | Homo sapiens | |
additional information | exogenous expression of Sp1/Sp3 increases Axl promoter activity | Drosophila sp. (in: flies) | |
additional information | two sites (Sp a and Sp b) proximal to the translation start site are indispensable for Axl promoter activity, whereas mutation of three additional upstream motifs (Sp c, Sp d and Sp e) is of additional relevance | Homo sapiens |
Application | Comment | Organism |
---|---|---|
medicine | Axl gene expression in cancer cells is constitutively driven by Sp1/Sp3 bound to five core promoter motifs, and is restricted by methylation within/around Sp-binding sites | Homo sapiens |
Protein Variants | Comment | Organism |
---|---|---|
additional information | mutation of all motifs abolishes binding of Sp1 and Sp3 | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
mithramycin | inhibits binding of Sp factors to GC-rich sites, dramatically reduces Axl promoter activity and Axl, Sp1 and Sp3 expression | Homo sapiens | |
siRNA | use of Sp1/Sp3 siRNAs significantly reducs Axl promoter activity and protein levels in Rko and HeLa cells | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila sp. (in: flies) | - |
- |
- |
Homo sapiens | P30530 | - |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
HCT-116 cell | - |
Homo sapiens | - |
HeLa cell | - |
Homo sapiens | - |
additional information | Colo-206F/WiDr cell | Homo sapiens | - |
additional information | SCHNEIDER SL2-cell | Drosophila sp. (in: flies) | - |
RKO cell | - |
Homo sapiens | - |
Synonyms | Comment | Organism |
---|---|---|
Axl | - |
Drosophila sp. (in: flies) |
Axl | - |
Homo sapiens |
receptor tyrosine kinase | - |
Drosophila sp. (in: flies) |
receptor tyrosine kinase | - |
Homo sapiens |