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Literature summary for 2.7.1.B20 extracted from
- A novel nucleoside kinase from Burkholderia thailandensis: a member of the phosphofructokinase B-type family of enzymes (2008), FEBS J., 275, 5865-5872.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| functionally overexpressed in Rhodococcus erythropolis | Burkholderia thailandensis |
Inhibitors
| Inhibitors | Comment | Organism | Structure |
|---|---|---|---|
| adenosine | concentrations above 0.1 mM lead to reduction in enzyme activity | Burkholderia thailandensis |  |
| Inosine | concentrations above 0.1 mM lead to reduction in enzyme activity | Burkholderia thailandensis | |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.02 | - |
adenosine | pH 7.0, 37°C | Burkholderia thailandensis | |
| 0.15 | - |
Inosine | pH 7.0, 37°C | Burkholderia thailandensis | |
| 0.46 | - |
guanosine | pH 7.0, 37°C | Burkholderia thailandensis | |
| 0.6 | 3 | ATP | pH 7.0, 37°C | Burkholderia thailandensis | |
| 0.79 | - |
ribavirin | pH 7.0, 37°C | Burkholderia thailandensis | |
| 0.87 | - |
mizoribine | pH 7.0, 37°C | Burkholderia thailandensis | |
| 1.1 | - |
2'-deoxyadenosine | pH 7.0, 37°C | Burkholderia thailandensis | |
| 1.7 | - |
uridine | pH 7.0, 37°C | Burkholderia thailandensis | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Co2+ | enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+ | Burkholderia thailandensis | |
| Mg2+ | enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+. Maximum activity is attained when the ATP/Mg2+ concentration ratio is 0.5 | Burkholderia thailandensis | |
| Ni2+ | enzyme activity requires the presence of divalent cations. Mn2+ is the most efficient, followed by Mg2+, Ni2+ and Co2+ | Burkholderia thailandensis | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 34000 | - |
2 * 34000, SDS-PAGE | Burkholderia thailandensis |
| 64000 | - |
gel filtration | Burkholderia thailandensis |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Burkholderia thailandensis | Q2SZE4 | - |
- |
| Burkholderia thailandensis DSM 13276 | Q2SZE4 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
- |
Burkholderia thailandensis |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 2'-deoxyadenosine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + 2'-deoxyadenosine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis DSM 13276 | ADP + 2'-deoxyadenosine 5'-phosphate | - |
? | |
| ATP + adenosine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | ADP + adenosine 5'-phosphate | - |
? | |
| ATP + adenosine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis DSM 13276 | ADP + adenosine 5'-phosphate | - |
? | |
| ATP + guanosine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | ADP + guanosine 5'-phosphate | - |
? | |
| ATP + inosine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | ADP + inosine 5'-phosphate | - |
? | |
| ATP + mizoribine | i.e. 5-hydroxy-1-beta-D-ribofuranosyl-1H-imidazole-4-carboxamide. Phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | ADP + mizoribine 5'-phosphate | - |
? | |
| ATP + ribavirin | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | ADP + ribavirin 5'-phosphate | - |
? | |
| ATP + ribavirin | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis DSM 13276 | ADP + ribavirin 5'-phosphate | - |
? | |
| ATP + uridine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | ADP + uridine 5'-phosphate | - |
? | |
| ATP + uridine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis DSM 13276 | ADP + uridine 5'-phosphate | - |
? | |
| CTP + inosine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | CDP + inosine 5'-phosphate | - |
? | |
| GTP + inosine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | GDP + inosine 5'-phosphate | - |
? | |
| ITP + inosine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | IDP + inosine 5'-phosphate | - |
? | |
| additional information | in the presence of D-ribose, L-ribose, fructose, glucose, 2-deoxy-glucose, xanthosine, galactose, xylose, N-acetyl glucosamine, fructose-6-phosphate, mannose-6-phosphate and ribose-5-phosphate, the activity is below the detection limit. No activity with cytidine or thamidine | Burkholderia thailandensis | ? | - |
? | |
| additional information | in the presence of D-ribose, L-ribose, fructose, glucose, 2-deoxy-glucose, xanthosine, galactose, xylose, N-acetyl glucosamine, fructose-6-phosphate, mannose-6-phosphate and ribose-5-phosphate, the activity is below the detection limit. No activity with cytidine or thamidine | Burkholderia thailandensis DSM 13276 | ? | - |
? | |
| TTP + inosine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | TDP + inosine 5'-phosphate | - |
? | |
| UTP + inosine | phosphate donor specificity in the order of relaltive velocity: ITP (200%), TTP (130%), GTP (125%), ATP (100%), CTP (45%), UTP (40%) | Burkholderia thailandensis | UDP + inosine 5'-phosphate | - |
? | |
Subunits
| Subunits | Comment | Organism |
|---|---|---|
| homodimer | 2 * 34000, SDS-PAGE | Burkholderia thailandensis |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| BTH_I1158 | locus name | Burkholderia thailandensis |
| nucleoside kinase | - |
Burkholderia thailandensis |
Temperature Stability [°C]
| Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 35 | - |
pH 7.0, 20 min, stable | Burkholderia thailandensis |
| 60 | - |
pH 7.0, 20 min, 60% loss of activity | Burkholderia thailandensis |
| 70 | - |
pH 7.0, 20 min, complete inactivation | Burkholderia thailandensis |
pH Stability
| pH Stability | pH Stability Maximum | Comment | Organism |
|---|---|---|---|
| 6 | 10 | 37°C, 3 h, stable | Burkholderia thailandensis |
kcat/KM [mM/s]
| kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.04 | - |
mizoribine | pH 7.0, 37°C | Burkholderia thailandensis | |
| 0.08 | - |
ribavirin | pH 7.0, 37°C | Burkholderia thailandensis | |
| 0.24 | - |
guanosine | pH 7.0, 37°C | Burkholderia thailandensis | |
| 0.28 | - |
uridine | pH 7.0, 37°C | Burkholderia thailandensis | |
| 0.84 | - |
2'-deoxyadenosine | pH 7.0, 37°C | Burkholderia thailandensis | |
| 6.05 | - |
adenosine | pH 7.0, 37°C | Burkholderia thailandensis | |
| 16 | - |
ATP | pH 7.0, 37°C | Burkholderia thailandensis | |
| 80 | - |
Inosine | pH 7.0, 37°C | Burkholderia thailandensis | |
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