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Literature summary for 2.7.1.91 extracted from

  • Park, C.G.; Lee, H.S.; Lee, H.Y.; Park, C.S.; Choi, O.H.
    Cloning and characterization of rat sphingosine kinase 1 with an N-terminal extension (2007), Biochem. Biophys. Res. Commun., 364, 702-707.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
cloning and characterization of an isoform of rat SK1 with an N-terminal extension (long-rSK1). When the long-rSK1 is transfected in COS-7 cells, SK activity is 53fold increased. Substrate specificity and dependency on divalent cations of long-rSK1 are similar to those of rSK1 Rattus norvegicus

Inhibitors

Inhibitors Comment Organism Structure
EDTA
-
Rattus norvegicus
N,N-dimethylsphingosine competitive inhibition with D-erythro-sphingosine as substrate Rattus norvegicus

Metals/Ions

Metals/Ions Comment Organism Structure
Ca2+ both isoforms (longrSK1 and rSK1) require divalent cations for their activity, both enzymes show the highest activity with Mg2+, followed by Mn2+ and Ca2+ Rattus norvegicus
Mg2+ both isoforms (longrSK1 and rSK1) require divalent cations for their activity, both enzymes show the highest activity with Mg2+, followed by Mn2+ and Ca2+ Rattus norvegicus
Mn2+ both isoforms (longrSK1 and rSK1) require divalent cations for their activity, both enzymes show the highest activity with Mg2+, followed by Mn2+ and Ca2+ Rattus norvegicus

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
49800
-
x * 49800, calculated from sequence Rattus norvegicus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Rattus norvegicus long-rSK1 is involved in cell signaling ?
-
?

Organism

Organism UniProt Comment Textmining
Rattus norvegicus Q1HGK5
-
-

Source Tissue

Source Tissue Comment Organism Textmining
brain expression of long-rSK1 mRNA Rattus norvegicus
-
heart expression of long-rSK1 mRNA, no expression of rSK1 mRNA Rattus norvegicus
-
kidney expression of long-rSK1 mRNA Rattus norvegicus
-
liver expression of long-rSK1 mRNA, no expression of rSK1 mRNA Rattus norvegicus
-
lung expression of long-rSK1 mRNA Rattus norvegicus
-
spleen expression of long-rSK1 mRNA Rattus norvegicus
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + D-erythro-dihydrosphingosine the activity of longrSK1 towards dihydrosphingosine is about 3.6fold higher than that of rSK1 Rattus norvegicus ADP + D-erythro-dihydrosphingosine 1-phosphate
-
?
ATP + D-erythro-sphingosine
-
Rattus norvegicus ADP + D-erythro-sphingosine 1-phosphate
-
?
additional information long-rSK1 is involved in cell signaling Rattus norvegicus ?
-
?
additional information no phosphorylation of with threo-dihydrosphingosine, N,N-dimethylsphingosine and N-acetyl-D-sphingosine Rattus norvegicus ?
-
?

Subunits

Subunits Comment Organism
? x * 49800, calculated from sequence Rattus norvegicus

Synonyms

Synonyms Comment Organism
SK1
-
Rattus norvegicus
sphingosine kinase 1
-
Rattus norvegicus

Ki Value [mM]

Ki Value [mM] Ki Value maximum [mM] Inhibitor Comment Organism Structure
0.00033
-
N,N-dimethylsphingosine rSK1 Rattus norvegicus
0.0058
-
N,N-dimethylsphingosine long-rSK1 Rattus norvegicus