General Stability | Organism |
---|---|
ATP protects against heat denaturation and tryptic digestion | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
diphosphate | - |
Rattus norvegicus | |
EDTA | - |
Rattus norvegicus | |
p-hydroxymercuribenzoate | 2-mercaptoethanol prevents inhibition | Rattus norvegicus | |
phosphate | - |
Rattus norvegicus | |
sulfate | - |
Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00069 | - |
ATP | pH 5.5, 37°C | Rattus norvegicus | |
0.0039 | - |
5'-OH-DNA | pH 5.5, 37°C | Rattus norvegicus |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
chromatin | - |
Rattus norvegicus | 785 | - |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
divalent cations | absolutely required | Rattus norvegicus | |
Mg2+ | stimulates | Rattus norvegicus | |
Mn2+ | most effective divalent cation in activation, best at 1 mM | Rattus norvegicus | |
additional information | maximal activity at ionic strength of 135 mM | Rattus norvegicus | |
Zn2+ | less effective | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
79000 | - |
gel filtration | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Rattus norvegicus | - |
ADP + 5'-phospho-DNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
enzyme also has DNA 3'-phosphatase activity | - |
Purification (Comment) | Organism |
---|---|
39fold | Rattus norvegicus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
liver | - |
Rattus norvegicus | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | native and heat denatured DNA | Rattus norvegicus | ADP + 5'-phospho-DNA | - |
? | |
ATP + nucleoside-3'-monophosphate | - |
Rattus norvegicus | ADP + nucleoside-3',5'-diphosphate | - |
? | |
additional information | enzyme also has DNA 3'-phosphatase activity | Rattus norvegicus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
More | enzyme also possesses a 3'-phosphatase activity | Rattus norvegicus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Rattus norvegicus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
pH-optimum of 3'-phosphatase activity is 6.0 | Rattus norvegicus |
5.5 | - |
assay at | Rattus norvegicus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ATP | dependent on | Rattus norvegicus |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Rattus norvegicus | - |
- |
8.6 |