Literature summary for 2.7.1.78 extracted from

Dikfidan, A.; Loll, B.; Zeymer, C.; Magler, I.; Clausen, T.; Meinhart, A.
RNA specificity and regulation of catalysis in the eukaryotic polynucleotide kinase Clp1 (2014), Mol. Cell, 54, 975-986.

Search for more literature for 2.7.1.78

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli BL21(DE3)-RIL cells	Caenorhabditis elegans

Crystallization (Commentary)

Crystallization (Comment)	Organism
in complex with the nonhydrolysable ATP analog adenosine 5'-(beta,gamma-imido)triphosphate, sparse matrix sampling, using 100 mM Na2HPO4/KH2PO4 (pH 5.0-6.0), 200 mM NaCl, 15 mM MgCl2, 90 mM sarcosine, and 25% (w/v) polyethylene glycol 1000	Caenorhabditis elegans

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.099	-	5'-dephospho-GAAAA-RNA	pH and temperature not specified in the publication	Caenorhabditis elegans
0.109	-	5'-dephospho-GC-RNA	pH and temperature not specified in the publication	Caenorhabditis elegans
0.259	-	ATP	pH and temperature not specified in the publication	Caenorhabditis elegans

Organism

Organism	UniProt	Comment	Textmining
Caenorhabditis elegans	P52874	-	-

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
ATP + 5'-dephospho-GAAAA-RNA	-	Caenorhabditis elegans	ADP + 5'-phospho-GAAAA-RNA	-	?
ATP + 5'-dephospho-GC-RNA	-	Caenorhabditis elegans	ADP + 5'-phospho-GC-RNA	-	?
additional information	the enzyme displays no significant activity on mononucleotides	Caenorhabditis elegans	?	-	?

Synonyms

Synonyms	Comment	Organism
CLP1	-	Caenorhabditis elegans
RNA-specific polynucleotide kinase	-	Caenorhabditis elegans
RPNK	-	Caenorhabditis elegans

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
2.6	-	5'-dephospho-GAAAA-RNA	pH and temperature not specified in the publication	Caenorhabditis elegans
2.9	-	ATP	pH and temperature not specified in the publication	Caenorhabditis elegans
2.9	-	5'-dephospho-GC-RNA	pH and temperature not specified in the publication	Caenorhabditis elegans

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Release 2023.1 (January 2023)