Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli C41(DE3) cells | Tequatrovirus T4 |
Protein Variants | Comment | Organism |
---|---|---|
G14D | the mutation impairs the 5`-kinase activity of the enzyme in vivo as well as in vitro and leads to diminished processing at secondary sites of several RegB-cleaved transcripts | Tequatrovirus T4 |
R229H | the mutant shows slightly reduced wild type activity | Tequatrovirus T4 |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | Tequatrovirus T4 | - |
ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | Tequatrovirus T4 K10 | - |
ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-RNA | Tequatrovirus T4 | - |
ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | Tequatrovirus T4 K10 | - |
ADP + 5'-phospho-RNA | - |
? | |
additional information | Tequatrovirus T4 | the enzyme catalyzes both, the phosphorylation of 5-hydroxyl termini and the hydrolysis of 3-phosphomonoesters and 2,3-cyclic phosphodiesters of polynucleotides | ? | - |
? | |
additional information | Tequatrovirus T4 K10 | the enzyme catalyzes both, the phosphorylation of 5-hydroxyl termini and the hydrolysis of 3-phosphomonoesters and 2,3-cyclic phosphodiesters of polynucleotides | ? | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tequatrovirus T4 | - |
- |
- |
Tequatrovirus T4 K10 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
His-Spin Protein Miniprep column chromatography, and gel filtration | Tequatrovirus T4 |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dephospho-DNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-DNA | - |
Tequatrovirus T4 K10 | ADP + 5'-phospho-DNA | - |
? | |
ATP + 5'-dephospho-RNA | - |
Tequatrovirus T4 | ADP + 5'-phospho-RNA | - |
? | |
ATP + 5'-dephospho-RNA | - |
Tequatrovirus T4 K10 | ADP + 5'-phospho-RNA | - |
? | |
additional information | the enzyme catalyzes both, the phosphorylation of 5-hydroxyl termini and the hydrolysis of 3-phosphomonoesters and 2,3-cyclic phosphodiesters of polynucleotides | Tequatrovirus T4 | ? | - |
? | |
additional information | the enzyme catalyzes both, the phosphorylation of 5-hydroxyl termini and the hydrolysis of 3-phosphomonoesters and 2,3-cyclic phosphodiesters of polynucleotides | Tequatrovirus T4 K10 | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
PNK | - |
Tequatrovirus T4 |