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Literature summary for 2.7.1.78 extracted from

  • Durand, S.; Richard, G.; Bontems, F.; Uzan, M.
    Bacteriophage T4 polynucleotide kinase triggers degradation of mRNAs (2012), Proc. Natl. Acad. Sci. USA, 109, 7073-7078.
    View publication on PubMedView publication on EuropePMC
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Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dephospho-RNA Tequatrovirus T4
-
 ADP + 5'-phospho-RNA
-
 ?

Organism

Organism UniProt Comment Textmining
Tequatrovirus T4
-
-
-

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dephospho-RNA
-
 Tequatrovirus T4 ADP + 5'-phospho-RNA
-
 ?

Synonyms

Synonyms Comment Organism
PNK
-
 Tequatrovirus T4
T4 PNK
-
 Tequatrovirus T4
T4 polynucleotide kinase/phosphatase
-
 Tequatrovirus T4

General Information

General Information Comment Organism
physiological function degradation of RegB-cleaved mRNAs depends on a functional T4 polynucleotide kinase/phosphatase. PNK controls the decay of early transcripts predominantly from their 5'-termini. The 5'-OH produced by RegB cleavage is phosphorylated by the kinase activity of PNK. When the 5'-OH RNA end generated by RegB is not phosphorylated by PNK, the attack by RNases E and G is blocked or decreased over a distance of about 300 nt from the RegB site. But after PNK has modified the 5'-terminus and RNase G (or E) has cut at secondary sites, the new 5'-monophosphorylated RNA ends can presumably activate RNases E and G in cascade. The PNK-dependent pathway of degradation becomes effective 5 min postinfectio. The T4 PNK also has a role during normal phage development Tequatrovirus T4