Cloned (Comment) | Organism |
---|---|
expression of GST-tagged human Nol9 in Spodoptera frugiperda Sf9 insect cells | Homo sapiens |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dsRNA | Homo sapiens | - |
ADP + 5'-phospho-dsRNA | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant GST-tagged human Nol9 from Sf9 insect cells by glutathione affinity chromatography | Homo sapiens |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ATP + 5'-dsRNA | - |
Homo sapiens | ADP + 5'-phospho-dsRNA | - |
? | |
additional information | recombinant Nol9 phosphorylates single-stranded and double-stranded RNA and DNA substrates with high efficiency, mainly pre-60S rRNP particles. Nol9 is able to transfer a phosphate to 5' ends of dsRNAs, but cannot phosphorylate 3' termini | Homo sapiens | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
5' end-specific RNA/DNA kinase | - |
Homo sapiens |
Nol9 | - |
Homo sapiens |
General Information | Comment | Organism |
---|---|---|
malfunction | depletion of Nol9 leads to a severe impairment of ribosome biogenesis. Upon Nol9 knockdown, specific maturation defect at the 5' end of the predominant 5.8S short-form rRNA (5.8SS) occur, possibly due to the Nol9 requirement for 5'>3' exonucleolytic trimming | Homo sapiens |
metabolism | polynucleotide 5-kinase Nol9 is involved in ribosomal RNA processing | Homo sapiens |
physiological function | the nonribosomal protein Nol9 is a polynucleotide 5'-kinase that sediments primarily with the pre-60S and pre-40S ribosomal particles in HeLa nuclear extracts. The polynucleotide kinase activity of Nol9 is required for processing of large subunit rRNA and efficient generation of the 5.8S and 28S rRNAs from the 32S precursor | Homo sapiens |