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Literature summary for 2.7.1.78 extracted from

  • Heindl, K.; Martinez, J.
    Nol9 is a novel polynucleotide 5-kinase involved in ribosomal RNA processing (2010), EMBO J., 29, 4161-4171.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
expression of GST-tagged human Nol9 in Spodoptera frugiperda Sf9 insect cells Homo sapiens

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
ATP + 5'-dsRNA Homo sapiens
-
ADP + 5'-phospho-dsRNA
-
?

Organism

Organism UniProt Comment Textmining
Homo sapiens
-
-
-

Purification (Commentary)

Purification (Comment) Organism
recombinant GST-tagged human Nol9 from Sf9 insect cells by glutathione affinity chromatography Homo sapiens

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
ATP + 5'-dsRNA
-
Homo sapiens ADP + 5'-phospho-dsRNA
-
?
additional information recombinant Nol9 phosphorylates single-stranded and double-stranded RNA and DNA substrates with high efficiency, mainly pre-60S rRNP particles. Nol9 is able to transfer a phosphate to 5' ends of dsRNAs, but cannot phosphorylate 3' termini Homo sapiens ?
-
?

Synonyms

Synonyms Comment Organism
5' end-specific RNA/DNA kinase
-
Homo sapiens
Nol9
-
Homo sapiens

General Information

General Information Comment Organism
malfunction depletion of Nol9 leads to a severe impairment of ribosome biogenesis. Upon Nol9 knockdown, specific maturation defect at the 5' end of the predominant 5.8S short-form rRNA (5.8SS) occur, possibly due to the Nol9 requirement for 5'>3' exonucleolytic trimming Homo sapiens
metabolism polynucleotide 5-kinase Nol9 is involved in ribosomal RNA processing Homo sapiens
physiological function the nonribosomal protein Nol9 is a polynucleotide 5'-kinase that sediments primarily with the pre-60S and pre-40S ribosomal particles in HeLa nuclear extracts. The polynucleotide kinase activity of Nol9 is required for processing of large subunit rRNA and efficient generation of the 5.8S and 28S rRNAs from the 32S precursor Homo sapiens