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Literature summary for 2.7.1.78 extracted from
- Characterization of a thermostable archaeal polynucleotide kinase homologous to human Clp1 (2009), RNA, 15, 923-931.
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| into the vector pET28-His10Smt3 for expression in Escherichia coli BL21DE3 cells | Pyrococcus horikoshii |
Protein Variants
| Protein Variants | Comment | Organism |
|---|---|---|
| D73A | mutation abolishes activity | Pyrococcus horikoshii |
| K49A | mutation reduces activity to 1% of the wild-type value | Pyrococcus horikoshii |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.016 | - |
5'-OH DNA | - |
Pyrococcus horikoshii |  |
| 0.021 | - |
5'-phospho-DNA | - |
Pyrococcus horikoshii | |
Metals/Ions
| Metals/Ions | Comment | Organism | Structure |
|---|---|---|---|
| Mg2+ | - |
Pyrococcus horikoshii | |
Molecular Weight [Da]
| Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
|---|---|---|---|
| 41000 | - |
determined by SDS-PAGE, tag-free | Pyrococcus horikoshii |
Natural Substrates/ Products (Substrates)
| Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-OH DNA | Pyrococcus horikoshii | - |
ADP + 5'-phospho-DNA | - |
r | |
| ATP + 5'-OH RNA | Pyrococcus horikoshii | preference for RNA substrates | ADP + 5'-phospho-RNA | - |
r | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Pyrococcus horikoshii | Q57936 | - |
- |
Purification (Commentary)
| Purification (Comment) | Organism |
|---|---|
| on a Ni-NTA-agarose column, the His10Smt3 tag is removed with the Smt3-specific protease Ulp1 | Pyrococcus horikoshii |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| ATP + 5'-OH DNA | - |
Pyrococcus horikoshii | ADP + 5'-phospho-DNA | - |
r | |
| ATP + 5'-OH RNA | preference for RNA substrates | Pyrococcus horikoshii | ADP + 5'-phospho-RNA | - |
r | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| 5'-OH polynucleotide kinase | - |
Pyrococcus horikoshii |
| Clp1-like protein | - |
Pyrococcus horikoshii |
| PhoClp1 | - |
Pyrococcus horikoshii |
| polynucleotide kinase | - |
Pyrococcus horikoshii |
Temperature Optimum [°C]
| Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | - |
kinase assay | Pyrococcus horikoshii |
Temperature Range [°C]
| Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
|---|---|---|---|
| 55 | 85 | - |
Pyrococcus horikoshii |
Turnover Number [1/s]
| Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.00083 | - |
5'-phospho-DNA | - |
Pyrococcus horikoshii | |
| 0.077 | - |
5'-OH DNA | - |
Pyrococcus horikoshii | |
pH Optimum
| pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
|---|---|---|---|
| 5 | - |
reverse reaction | Pyrococcus horikoshii |
| 6.5 | - |
kinase assay | Pyrococcus horikoshii |
| 7.5 | - |
kinase assay | Pyrococcus horikoshii |
pH Range
| pH Minimum | pH Maximum | Comment | Organism |
|---|---|---|---|
| 4.5 | 9.5 | DNA kinase activity | Pyrococcus horikoshii |
| 4.5 | 6.5 | RNA kinase activity | Pyrococcus horikoshii |
Cofactor
| Cofactor | Comment | Organism | Structure |
|---|---|---|---|
| ATP | - |
Pyrococcus horikoshii | |
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Release 2023.1 (January 2023)
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